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烟酰胺腺嘌呤二核苷酸和三磷酸腺苷在糖皮质激素诱导的易感淋巴细胞细胞毒性中的作用。

Role of nicotinamide adenine dinucleotide and adenosine triphosphate in glucocorticoid-induced cytotoxicity in susceptible lymphoid cells.

作者信息

Berger N A, Berger S J, Sudar D C, Distelhorst C W

出版信息

J Clin Invest. 1987 Jun;79(6):1558-63. doi: 10.1172/JCI112989.

DOI:10.1172/JCI112989
PMID:3108318
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC424466/
Abstract

The possibility that corticosteroid cytotoxicity could be mediated by activation of poly(ADP-ribose) polymerase and consequent depletion of NAD and ATP was evaluated in steroid-sensitive S49.1 and steroid-resistant S49.143R mouse lymphoma cells and in lymphocytes from a patient with chronic lymphocytic leukemia. All cell types were shown to have the enzyme poly(ADP-ribose) polymerase and to increase activity in response to DNA strand breaks. Incubation of susceptible cells with 1 microM dexamethasone resulted in DNA strand breaks. Susceptible cells also showed a dose-dependent decrease in NAD and ATP that preceded loss of cell viability. These studies suggest that steroid-induced cytotoxicity in susceptible lymphocytes is due to the presence of DNA strand breaks that activate poly(ADP-ribose) polymerase to a sufficient degree to consume cellular pools of NAD with a consequent depletion of ATP and loss of cell viability.

摘要

在类固醇敏感的S49.1和类固醇耐药的S49.143R小鼠淋巴瘤细胞以及一名慢性淋巴细胞白血病患者的淋巴细胞中，评估了皮质类固醇细胞毒性可能由多聚（ADP - 核糖）聚合酶激活以及随后NAD和ATP耗竭介导的可能性。所有细胞类型均显示具有多聚（ADP - 核糖）聚合酶，并在响应DNA链断裂时增加活性。用1微摩尔地塞米松孵育敏感细胞会导致DNA链断裂。敏感细胞还显示出NAD和ATP的剂量依赖性降低，这在细胞活力丧失之前出现。这些研究表明，类固醇诱导的敏感淋巴细胞细胞毒性是由于存在DNA链断裂，其激活多聚（ADP - 核糖）聚合酶的程度足以消耗细胞内的NAD池，从而导致ATP耗竭和细胞活力丧失。

相似文献

1
Role of nicotinamide adenine dinucleotide and adenosine triphosphate in glucocorticoid-induced cytotoxicity in susceptible lymphoid cells.烟酰胺腺嘌呤二核苷酸和三磷酸腺苷在糖皮质激素诱导的易感淋巴细胞细胞毒性中的作用。
J Clin Invest. 1987 Jun;79(6):1558-63. doi: 10.1172/JCI112989.
2
Glucocorticoid-induced cell death and poly[adenosine diphosphate(ADP)-ribosyl]ation: increased toxicity of dexamethasone on mouse S49.1 lymphoma cells with the poly(ADP-ribosyl)ation inhibitor benzamide.糖皮质激素诱导的细胞死亡与多聚[腺苷二磷酸（ADP）-核糖基]化：多聚（ADP-核糖基）化抑制剂苯甲酰胺增强地塞米松对小鼠S49.1淋巴瘤细胞的毒性。
Endocrinology. 1986 Nov;119(5):2383-92. doi: 10.1210/endo-119-5-2383.
3
Oxidant injury of cells. DNA strand-breaks activate polyadenosine diphosphate-ribose polymerase and lead to depletion of nicotinamide adenine dinucleotide.细胞的氧化损伤。DNA链断裂激活聚腺苷二磷酸核糖聚合酶并导致烟酰胺腺嘌呤二核苷酸耗竭。
J Clin Invest. 1986 Apr;77(4):1312-20. doi: 10.1172/JCI112436.
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Radiat Res. 1985 Jan;101(1):4-15.
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Modulation of nicotinamide adenine dinucleotide and poly(adenosine diphosphoribose) metabolism by calicheamicin gamma 1 in human HL-60 cells.加利车霉素γ1对人HL-60细胞中烟酰胺腺嘌呤二核苷酸和聚（腺苷二磷酸核糖）代谢的调节作用
Cancer Lett. 1990 Apr 20;50(2):141-7. doi: 10.1016/0304-3835(90)90244-r.
6
(ADP-ribose)n participates in DNA excision repair.（ADP - 核糖）n参与DNA切除修复。
Nature. 1980 Feb 7;283(5747):593-6. doi: 10.1038/283593a0.
7
Modulation of nicotinamide adenine dinucleotide and poly(adenosine diphosphoribose) metabolism by the synthetic "C" nucleoside analogs, tiazofurin and selenazofurin. A new strategy for cancer chemotherapy.合成的“C”核苷类似物替拉扎明和硒代替拉扎明对烟酰胺腺嘌呤二核苷酸和聚（腺苷二磷酸核糖）代谢的调节作用。一种癌症化疗的新策略。
J Clin Invest. 1985 Feb;75(2):702-9. doi: 10.1172/JCI111750.
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Hydrogen peroxide insult in cultured mammalian cells: relationships between DNA single-strand breakage, poly(ADP-ribose) metabolism and cell killing.培养的哺乳动物细胞中过氧化氢损伤：DNA单链断裂、聚（ADP-核糖）代谢与细胞杀伤之间的关系。
Biochim Biophys Acta. 1989 Oct 30;1014(1):1-7. doi: 10.1016/0167-4889(89)90234-6.
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Effects of nicotinamide on NAD and poly(ADP-ribose) metabolism in DNA-damaged human lymphocytes.烟酰胺对DNA损伤的人淋巴细胞中NAD及聚（ADP - 核糖）代谢的影响
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Ex vivo supplementation with nicotinic acid enhances cellular poly(ADP-ribosyl)ation and improves cell viability in human peripheral blood mononuclear cells.体外补充烟酰胺酸可增强人外周血单核细胞的细胞多聚（ADP-核糖）化并提高细胞活力。
Biochem Pharmacol. 2010 Oct 1;80(7):1103-12. doi: 10.1016/j.bcp.2010.06.010. Epub 2010 Jun 25.

引用本文的文献

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Elevation of apoptotic potential by anoxia hyperoxia shift in NIH3T3 cells.NIH3T3细胞中缺氧-高氧转换导致凋亡潜能升高。
Mol Cell Biochem. 1999 Jul;197(1-2):147-59. doi: 10.1023/a:1006941630901.
2
The role of phosphometabolites in cell proliferation, energy metabolism, and tumor therapy.磷酸代谢物在细胞增殖、能量代谢及肿瘤治疗中的作用。
J Bioenerg Biomembr. 1997 Aug;29(4):315-30. doi: 10.1023/a:1022490512705.
3
Programmed cell death--many questions still to be answered.程序性细胞死亡——仍有许多问题有待解答。
Ann Hematol. 1994 Aug;69(2):45-55. doi: 10.1007/BF01698481.
4
Apoptosis induced by anticancer drugs.
Cancer Metastasis Rev. 1992 Sep;11(2):121-39. doi: 10.1007/BF00048059.

本文引用的文献

1
Effect of cell cycle position on dexamethasone binding by mouse and human lymphoid cell lines: correlation between an increase in dexamethasone binding during S phase and dexamethasone sensitivity.细胞周期位置对小鼠和人淋巴样细胞系地塞米松结合的影响：S期地塞米松结合增加与地塞米松敏感性之间的相关性。
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Variation in normal and tumor tissue sensitivity of mice to ionizing radiation-induced DNA strand breaks in vivo.小鼠正常组织和肿瘤组织对体内电离辐射诱导的DNA链断裂的敏感性差异。
Cancer Res. 1983 Dec;43(12 Pt 1):5668-73.
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Human B lymphocytes show greater susceptibility to H2O2 toxicity than T lymphocytes.人类B淋巴细胞比T淋巴细胞对过氧化氢毒性更敏感。
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Inhibitors of poly-(adenosine diphosphoribose) synthesis slow the resealing rate of x-ray-induced DNA strand breaks.
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Glucocorticoid activation of a calcium-dependent endonuclease in thymocyte nuclei leads to cell death.糖皮质激素激活胸腺细胞核中一种钙依赖性核酸内切酶会导致细胞死亡。
J Immunol. 1984 Jan;132(1):38-42.
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Poly(ADP-ribose) Polymerase inhibitors preserve nicotinamide adenine dinucleotide and adenosine 5'-triphosphate pools in DNA-damaged cells: mechanism of stimulation of unscheduled DNA synthesis.聚（ADP-核糖）聚合酶抑制剂可维持DNA损伤细胞中的烟酰胺腺嘌呤二核苷酸和三磷酸腺苷水平：刺激非预定DNA合成的机制
Biochemistry. 1983 Oct 25;22(22):5188-94. doi: 10.1021/bi00291a019.
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Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation.糖皮质激素诱导的胸腺细胞凋亡与内源性核酸内切酶激活有关。
Nature. 1980 Apr 10;284(5756):555-6. doi: 10.1038/284555a0.
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(ADP-ribose)n participates in DNA excision repair.（ADP - 核糖）n参与DNA切除修复。
Nature. 1980 Feb 7;283(5747):593-6. doi: 10.1038/283593a0.
10
Glucocorticoid receptors in lymphoma cells in culture: relationship to glucocorticoid killing activity.培养的淋巴瘤细胞中的糖皮质激素受体：与糖皮质激素杀伤活性的关系。
Science. 1971 Jan 15;171(3967):189-91. doi: 10.1126/science.171.3967.189.