Hayes Peggy S, Graves Lewis M, Swaminathan B, Ajello Gloria W, Malcolm Georgia B, Weaver Robert E, Ransom Ray, Deaver Katherine, Plikaytis Brian D, Schuchat Anne, Wenger Jay D, Pinner Robert W, Broome Claire V
Meningitis and Special Pathogens Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
J Food Prot. 1992 Dec;55(12):952-959. doi: 10.4315/0362-028X-55.12.952.
Three selective enrichment procedures-the U.S. Food and Drug Administration (FDA) method, the U.S. Department of Agriculture (USDA) method, and the Netherlands Government Food Inspection Service (NGFIS) method-were compared for isolating Listeria monocytogenes from contaminated foods. The foods were obtained from the refrigerators of patients with culture-proven listeriosis who were identified through multistate active surveillance in a U.S. population of 19 million. The study was designed to identify foods that may be important in transmission of L. monocytogenes in sporadic cases of human listeriosis. Of 899 foods analyzed by all three methods, 121 were positive for L. monocytogenes by at least one method. The three enrichment methods detected L. monocytogenes in 65% (FDA), 74% (USDA), and 74% (NGFIS) of the foods shown to contain L. monocytogenes . The differences among the three methods were not statistically significant. However, the recovery of L. monocytogenes by a combination of any two methods (USDA-FDA 88%, USDA-NGFIS 91%, FDA-NGFIS 87%) was significantly better than that by one method alone (p < 0.02). The differences among the combinations of methods were not statistically significant. These results suggest that at least two enrichment methods must be used in combination to recover L. monocytogenes from contaminated foods with a success rate near 90%. Correlations were observed between negative results and low (<0.3 CFU/g) level of L. monocytogenes contamination for the USDA (p << 0.001) and NGFIS (p << 0.001) methods. A similar but somewhat weaker association was observed for the FDA method (p < 0.06).
比较了三种选择性富集程序——美国食品药品监督管理局（FDA）方法、美国农业部（USDA）方法和荷兰政府食品检验局（NGFIS）方法——用于从受污染食品中分离单核细胞增生李斯特菌。这些食品取自经培养证实患有李斯特菌病患者的冰箱，这些患者是通过对美国1900万人口进行的多州主动监测确定的。该研究旨在确定在人类散发性李斯特菌病病例中可能对单核细胞增生李斯特菌传播起重要作用的食品。在通过所有三种方法分析的899份食品中，有121份至少通过一种方法检测出单核细胞增生李斯特菌呈阳性。这三种富集方法在显示含有单核细胞增生李斯特菌的食品中检测到该菌的比例分别为65%（FDA）、74%（USDA）和74%（NGFIS）。这三种方法之间的差异无统计学意义。然而，任意两种方法联合使用时单核细胞增生李斯特菌的回收率（USDA - FDA为88%，USDA - NGFIS为91%，FDA - NGFIS为87%）显著高于单独使用一种方法（p < 0.02）。方法组合之间的差异无统计学意义。这些结果表明，必须至少联合使用两种富集方法才能以接近90% 的成功率从受污染食品中回收单核细胞增生李斯特菌。对于USDA方法（p << 0.001）和NGFIS方法（p << 0.001），阴性结果与单核细胞增生李斯特菌低污染水平（<0.3 CFU/g）之间存在相关性。对于FDA方法观察到类似但稍弱的关联（p < 0.06）。
