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1
Comparison of cold enrichment and U.S. Department of Agriculture methods for isolating Listeria monocytogenes from naturally contaminated foods. The Listeria Study Group.用于从天然污染食品中分离单核细胞增生李斯特菌的冷增菌法与美国农业部方法的比较。李斯特菌研究小组。
Appl Environ Microbiol. 1991 Aug;57(8):2109-13. doi: 10.1128/aem.57.8.2109-2113.1991.
2
Comparison of Three Selective Enrichment Methods for the Isolation of Listeria monocytogenes from Naturally Contaminated Foods.从天然污染食品中分离单核细胞增生李斯特菌的三种选择性富集方法的比较
J Food Prot. 1992 Dec;55(12):952-959. doi: 10.4315/0362-028X-55.12.952.
3
Role of foods in sporadic listeriosis. II. Microbiologic and epidemiologic investigation. The Listeria Study Group.食品在散发性李斯特菌病中的作用。II. 微生物学和流行病学调查。李斯特菌研究小组。
JAMA. 1992 Apr 15;267(15):2046-50.
4
Enrichment dynamics of Listeria monocytogenes and the associated microbiome from naturally contaminated ice cream linked to a listeriosis outbreak.与一次李斯特菌病暴发相关的、来自天然污染冰淇淋的单核细胞增生李斯特菌及相关微生物群落的富集动态。
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Validation of the USDA/ARS package rinse method for recovery of Listeria monocytogenes from naturally contaminated, commercially prepared frankfurters.
J Food Prot. 2003 Oct;66(10):1920-3. doi: 10.4315/0362-028x-66.10.1920.
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Comparison of a cold enrichment method and the IDF method for isolation of Listeria monocytogenes from animal autopsy material.
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Relatedness of Listeria monocytogenes Isolates recovered from selected ready-to-eat foods and listeriosis patients in the United States.从美国部分即食食品和李斯特菌病患者中分离出的单核细胞增生李斯特菌菌株的相关性。
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Comparison of Listeria monocytogenes recoveries from spiked mung bean sprouts by the enrichment methods of three regulatory agencies.三种监管机构的增菌方法对豆芽中李斯特菌回收效果的比较。
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Comparison of a cold enrichment and the FDA method for isolating Listeria monocytogenes and other Listeria spp. from ready-to-eat food on retail sale in the U.K.
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Subtyping of Listeria monocytogenes isolates recovered from retail ready-to-eat foods, processing plants and listeriosis patients in Sweden 2010.2010 年瑞典从零售即食食品、加工厂和李斯特菌病患者中分离出的单核细胞增生李斯特菌分离株的分型。
Int J Food Microbiol. 2013 Aug 16;166(1):186-92. doi: 10.1016/j.ijfoodmicro.2013.06.008. Epub 2013 Jun 24.

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Listeriosis in human pregnancy: a systematic review.人类妊娠李斯特菌病：系统评价。
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Estimation of microbial contamination of food from prevalence and concentration data: application to Listeria monocytogenes in fresh vegetables.根据流行率和浓度数据估算食品中的微生物污染：应用于新鲜蔬菜中的单核细胞增生李斯特菌
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7
Microbiological aspects of the investigation that traced the 1998 outbreak of listeriosis in the United States to contaminated hot dogs and establishment of molecular subtyping-based surveillance for Listeria monocytogenes in the PulseNet network.一项调查的微生物学情况，该调查追踪到美国1998年由受污染热狗引发的李斯特菌病疫情，并在脉冲网络中建立了基于分子分型的单核细胞增生李斯特菌监测。
J Clin Microbiol. 2005 May;43(5):2350-5. doi: 10.1128/JCM.43.5.2350-2355.2005.
8
Update on Listeria monocytogenes infection.单核细胞增生李斯特菌感染的最新情况。
Curr Gastroenterol Rep. 2002 Aug;4(4):287-96. doi: 10.1007/s11894-002-0078-z.
9
Detection of viable Listeria monocytogenes with a 5' nuclease PCR assay.用5'核酸酶聚合酶链反应检测单核细胞增生李斯特菌活菌。
Appl Environ Microbiol. 1999 May;65(5):2122-7. doi: 10.1128/AEM.65.5.2122-2127.1999.
10
Differentiation of epidemic-associated strains of Listeria monocytogenes by restriction fragment length polymorphism in a gene region essential for growth at low temperatures (4 degrees C).通过低温（4℃）生长必需基因区域的限制性片段长度多态性对单核细胞增生李斯特菌的疫情相关菌株进行鉴别。
Appl Environ Microbiol. 1995 Dec;61(12):4310-4. doi: 10.1128/aem.61.12.4310-4314.1995.

本文引用的文献

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A New Technique for Isolating Listerellae from the Bovine Brain.一种从牛脑分离李斯特菌的新技术。
J Bacteriol. 1948 Apr;55(4):471-6.
2
New methodology for the isolation of Listeria microorganisms from heavily contaminated environments.从严重污染环境中分离李斯特菌微生物的新方法。
Appl Environ Microbiol. 1984 May;47(5):1188-90. doi: 10.1128/aem.47.5.1188-1190.1984.
3
Epidemic listeriosis--evidence for transmission by food.流行性李斯特菌病——通过食物传播的证据。
N Engl J Med. 1983 Jan 27;308(4):203-6. doi: 10.1056/NEJM198301273080407.
4
Isolation and enumeration of Listeria monocytogenes from Sewage, Sewage Sludge and River Water.
J Appl Bacteriol. 1981 Feb;50(1):1-9. doi: 10.1111/j.1365-2672.1981.tb00865.x.
5
Isolation of Listeria monocytogenes from faeces of clinically healthy humans and animals.从临床健康的人和动物粪便中分离出单核细胞增生李斯特菌。
Zentralbl Bakteriol Orig. 1969;211(3):353-9.
6
Isolation of Listeria monocytogenes from vegetation.从植被中分离单核细胞增生李斯特菌。
J Bacteriol. 1968 Feb;95(2):300-3. doi: 10.1128/jb.95.2.300-303.1968.
7
Wild birds and silage as reservoirs of Listeria in the agricultural environment.野生鸟类和青贮饲料作为农业环境中李斯特菌的储存宿主。
J Appl Bacteriol. 1985 Dec;59(6):537-43. doi: 10.1111/j.1365-2672.1985.tb03357.x.
8
Pasteurized milk as a vehicle of infection in an outbreak of listeriosis.巴氏杀菌乳作为李斯特菌病暴发中的感染载体。
N Engl J Med. 1985 Feb 14;312(7):404-7. doi: 10.1056/NEJM198502143120704.
9
A new selective medium for isolating Listeria spp. from heavily contaminated material.一种用于从严重污染材料中分离李斯特菌属的新型选择性培养基。
Appl Environ Microbiol. 1988 Jan;54(1):165-7. doi: 10.1128/aem.54.1.165-167.1988.
10
Evaluation of selective direct plating media for their suitability to recover uninjured, heat-injured, and freeze-injured Listeria monocytogenes from foods.评估选择性直接平板培养基从食品中回收未受损、热损伤和冻损伤的单核细胞增生李斯特菌的适用性。
Appl Environ Microbiol. 1988 Jun;54(6):1451-6. doi: 10.1128/aem.54.6.1451-1456.1988.

用于从天然污染食品中分离单核细胞增生李斯特菌的冷增菌法与美国农业部方法的比较。李斯特菌研究小组。

Comparison of cold enrichment and U.S. Department of Agriculture methods for isolating Listeria monocytogenes from naturally contaminated foods. The Listeria Study Group.

作者信息

Hayes P S, Graves L M, Ajello G W, Swaminathan B, Weaver R E, Wenger J D, Schuchat A, Broome C V

机构信息

Meningitis and Special Pathogens Branch, Centers for Disease Control, Atlanta, Georgia 30333.

出版信息

Appl Environ Microbiol. 1991 Aug;57(8):2109-13. doi: 10.1128/aem.57.8.2109-2113.1991.

DOI:10.1128/aem.57.8.2109-2113.1991
PMID:1768082
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC183536/
Abstract

We compared the cold enrichment (CE) and U.S. Department of Agriculture (USDA) methods for isolating Listeria monocytogenes by examining 402 food samples. The food samples were collected from refrigerators of listeriosis patients as part of a multistate active surveillance project to determine the role of foods in sporadic listeriosis in the United States. L. monocytogenes was isolated from 51 food samples (13%). The USDA method was significantly better (P less than 0.001) than the CE method. The isolation efficiencies of the USDA and CE methods were 96 and 59%, respectively. Quantitation of L. monocytogenes in the food samples revealed that many food samples containing less than 0.3 CFU/g were negative as determined by the CE method but positive as determined by the USDA method.

摘要

我们通过检测402份食品样本，比较了冷增菌法（CE）和美国农业部（USDA）分离单核细胞增生李斯特菌的方法。这些食品样本取自李斯特菌病患者的冰箱，是一项多州主动监测项目的一部分，该项目旨在确定食品在美国散发性李斯特菌病中的作用。从51份食品样本（13%）中分离出了单核细胞增生李斯特菌。USDA方法明显优于CE方法（P小于0.001）。USDA方法和CE方法的分离效率分别为96%和59%。对食品样本中单核细胞增生李斯特菌的定量分析表明，许多每克含菌量低于0.3 CFU的食品样本，用CE方法检测为阴性，但用USDA方法检测为阳性。