Hayes P S, Graves L M, Ajello G W, Swaminathan B, Weaver R E, Wenger J D, Schuchat A, Broome C V
Meningitis and Special Pathogens Branch, Centers for Disease Control, Atlanta, Georgia 30333.
Appl Environ Microbiol. 1991 Aug;57(8):2109-13. doi: 10.1128/aem.57.8.2109-2113.1991.
We compared the cold enrichment (CE) and U.S. Department of Agriculture (USDA) methods for isolating Listeria monocytogenes by examining 402 food samples. The food samples were collected from refrigerators of listeriosis patients as part of a multistate active surveillance project to determine the role of foods in sporadic listeriosis in the United States. L. monocytogenes was isolated from 51 food samples (13%). The USDA method was significantly better (P less than 0.001) than the CE method. The isolation efficiencies of the USDA and CE methods were 96 and 59%, respectively. Quantitation of L. monocytogenes in the food samples revealed that many food samples containing less than 0.3 CFU/g were negative as determined by the CE method but positive as determined by the USDA method.
我们通过检测402份食品样本,比较了冷增菌法(CE)和美国农业部(USDA)分离单核细胞增生李斯特菌的方法。这些食品样本取自李斯特菌病患者的冰箱,是一项多州主动监测项目的一部分,该项目旨在确定食品在美国散发性李斯特菌病中的作用。从51份食品样本(13%)中分离出了单核细胞增生李斯特菌。USDA方法明显优于CE方法(P小于0.001)。USDA方法和CE方法的分离效率分别为96%和59%。对食品样本中单核细胞增生李斯特菌的定量分析表明,许多每克含菌量低于0.3 CFU的食品样本,用CE方法检测为阴性,但用USDA方法检测为阳性。