Nichols F C, Garrison S W
J Interferon Res. 1987 Feb;7(1):121-9. doi: 10.1089/jir.1987.7.121.
Interferon-gamma (IFN-gamma) can act to potentiate lipopolysaccharide (LPS)-stimulated processes in mononuclear phagocytes, including interleukin-1 release and tumoricidal activity. The present investigation examined the capacity of IFN-gamma to modulate LPS-stimulated prostaglandin E2 (PGE2) and thromboxane B2 (TxB2) release from counterflow isolated human monocytes. The release of PGE2 and TxB2 was compared for cells incubated with IFN-gamma prior to treatment with LPS and for cells treated simultaneously with IFN-gamma and LPS. Treatment of cells with IFN-gamma prior to stimulation with LPS (10 micrograms/ml, Salmonella typhimurium) resulted in elevated prostaglandin E (by immunoassay) and [3H]PGE2 release from monocytes when compared with LPS-treated cultures. In contrast, IFN-gamma pretreatment did not potentiate labeled or immunoreactive TxB2 release from LPS-treated monocytes. IFN-gamma pretreatment without LPS stimulation did not result in elevated eicosanoid release over controls. In addition, continuous treatment of monocytes with both IFN-gamma and LPS did not result in greater release of PGE2 and TxB2 than the summed individual effects of IFN-gamma and LPS. These results indicate that IFN-gamma selectively potentiates LPS-stimulated arachidonic acid conversion to PGE2 and not TxB2 in human monocytes. This effect was observed only for monocytes pretreated with IFN-gamma prior to stimulation with LPS.
干扰素-γ（IFN-γ）可增强单核吞噬细胞中脂多糖（LPS）刺激的过程，包括白细胞介素-1释放和杀肿瘤活性。本研究检测了IFN-γ调节LPS刺激的逆流分离人单核细胞中前列腺素E2（PGE2）和血栓素B2（TxB2）释放的能力。比较了在LPS处理前用IFN-γ孵育的细胞以及同时用IFN-γ和LPS处理的细胞中PGE2和TxB2的释放情况。在用LPS（10微克/毫升，鼠伤寒沙门氏菌）刺激前用IFN-γ处理细胞，与LPS处理的培养物相比，单核细胞中前列腺素E（通过免疫测定）和[3H]PGE2释放增加。相反，IFN-γ预处理并未增强LPS处理的单核细胞中标记的或免疫反应性TxB2的释放。无LPS刺激的IFN-γ预处理不会导致类花生酸释放高于对照。此外，同时用IFN-γ和LPS持续处理单核细胞，PGE2和TxB2的释放量并不比IFN-γ和LPS单独作用的总和更大。这些结果表明，IFN-γ在人单核细胞中选择性地增强LPS刺激的花生四烯酸转化为PGE2，而不是TxB2。仅在用LPS刺激前用IFN-γ预处理的单核细胞中观察到这种效应。
