Nagai H, Yamada H, Matsuura N, Iwamoto T, Choi S H, Koda A
J Pharmacobiodyn. 1987 Jan;10(1):49-54. doi: 10.1248/bpb1978.10.49.
The release of slow reacting substance of anaphylaxis (SRS-A) by anti-immunoglobulin E(IgE; epsilon)-antibody mediated passive peritoneal anaphylaxis (PPA) in rats was investigated immunopharmacologically. A significant amount of SRS-A was released by anti-epsilon-antibody in the peritoneal cavity of rats passively sensitized with IgE. The amount of SRS-A released by anti-epsilon-antibody was about one third less than that released in an anti-gamma-antibody and IgG2a system. The release of SRS-A was initiated at 2 min and reached its maximum 5 to 10 min after the injection of anti-epsilon-antibody. Disodium cromoglycate, tranilast and ketotifen inhibited the release of both SRS-A and histamine caused by anti-epsilon-antibody mediated PPA. Glucocorticoids (hydrocortisone, prednisolone and dexamethasone) also inhibited the release of both mediators. rho-Bromophenacyl bromide inhibited the release of both mediators. AA-861, a potent 5-lipoxygenase inhibitor, inhibited the release of SRS-A but not histamine. Indomethacin slightly enhanced the release of SRS-A and inhibited the release of histamine. Cytarabine resulted in leucopenia and inhibited the release of histamine but not SRS-A during PPA. Dextran sulfate reduced the number of glass adherent peritoneal cells and inhibited the release of SRS-A but not histamine. These results suggest the suitability of anti-epsilon-antibody mediated rat PPA for investigating the effect of anti-allergic agents on the release of SRS-A.
采用免疫药理学方法研究了抗免疫球蛋白E（IgE；ε）抗体介导的大鼠被动腹膜过敏反应（PPA）中过敏反应慢反应物质（SRS-A）的释放情况。在用IgE被动致敏的大鼠腹腔中，抗ε抗体可释放出大量的SRS-A。抗ε抗体释放的SRS-A量比抗γ抗体和IgG2a系统释放的量少约三分之一。注射抗ε抗体后2分钟开始释放SRS-A，5至10分钟达到释放高峰。色甘酸钠、曲尼司特和酮替芬可抑制抗ε抗体介导的PPA引起的SRS-A和组胺的释放。糖皮质激素（氢化可的松、泼尼松龙和地塞米松）也可抑制这两种介质的释放。ρ-溴苯甲酰溴可抑制这两种介质的释放。强效5-脂氧合酶抑制剂AA-861可抑制SRS-A的释放，但不抑制组胺的释放。吲哚美辛可轻微增强SRS-A的释放并抑制组胺的释放。阿糖胞苷导致白细胞减少，并在PPA期间抑制组胺的释放，但不抑制SRS-A的释放。硫酸葡聚糖可减少玻璃黏附腹膜细胞的数量并抑制SRS-A的释放，但不抑制组胺的释放。这些结果表明，抗ε抗体介导的大鼠PPA适用于研究抗过敏药物对SRS-A释放的影响。
