Université Clermont Auvergne, INRA, UNH, Unité de Nutrition Humaine, CRNH Auvergne, Clermont-Ferrand, France.
Chair of Food Chemistry, Faculty of Mathematics and Natural Sciences, Gaußstraße 20, University of Wuppertal, 42119, Wuppertal, Germany.
Free Radic Biol Med. 2019 Nov 20;144:72-89. doi: 10.1016/j.freeradbiomed.2019.05.012. Epub 2019 May 11.
Oxylipins, including the well-known eicosanoids, are potent lipid mediators involved in numerous physiological and pathological processes. Therefore, their quantitative profiling has gained a lot of attention during the last years notably in the active field of health biomarker discovery. Oxylipins include hundreds of structurally and stereochemically distinct lipid species which today are most commonly analyzed by (ultra) high performance liquid chromatography-mass spectrometry based ((U)HPLC-MS) methods. To maximize the utility of oxylipin profiling in clinical research, it is crucial to understand and assess the factors contributing to the analytical and biological variability of oxylipin profiles in humans. In this review, these factors and their impacts are summarized and discussed, providing a framework for recommendations expected to enhance the interlaboratory comparability and biological interpretation of oxylipin profiling in clinical research.
氧化脂类，包括著名的类二十烷酸，是参与众多生理和病理过程的强效脂质介质。因此，在过去几年中，它们的定量分析引起了广泛关注，特别是在健康生物标志物发现的活跃领域。氧化脂类包括数百种结构和立体化学上不同的脂质种类，目前最常通过（超）高效液相色谱-质谱法（（U）HPLC-MS）进行分析。为了最大限度地提高氧化脂类谱分析在临床研究中的效用，了解和评估导致人类氧化脂类谱分析和生物学变异性的因素至关重要。在这篇综述中，总结和讨论了这些因素及其影响，为增强氧化脂类谱分析在临床研究中的实验室间可比性和生物学解释提供了一个框架。
