Zhang Yinghuan, Sun Yunxia, Sun Junpeng, Feng Hui, Wang Yugang
College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China.
Liaoing Dongya Seed Limited Company, Shenyang 110164, China.
Breed Sci. 2019 Mar;69(1):47-54. doi: 10.1270/jsbbs.18108. Epub 2019 Feb 8.
Seed coat color is an important agronomic trait in . Yellow seeds are a desirable trait for breeding oilseed crops. To identify quantitative trait loci (QTLs) that condition seed coat color in , we used a population of recombinant inbred lines (RILs) derived from crossing 09A001, a standard rapid-cycling (RcBr) inbred line of L. ssp. with yellow seeds, with 08A061, an inbred line of heading Chinese cabbage with dark brown seeds. Using two phenotypic scoring methods, we detected a total of nine QTLs distributed on four chromosomes (Chrs.), A03, A06, A08, and A09, that explained 3.17 to 55.73% of the phenotypic variation for seed color. To validate the effects of the identified QTLs in the RIL population, chromosome segment substitution lines (CSSLs) harboring the chromosomal segment carrying the candidate QTL region from 08A061 were selected, and two co-localized major QTLs, and , and one minor QTL, , were successfully validated. The validated QTL located on Chr. A03 appears to be a new locus underlying seed coat color in . These findings provide additional insight that will help explain the complex genetic mechanisms underlying the seed coat color trait in .
种皮颜色是[作物名称]中的一个重要农艺性状。黄色种子是油料作物育种中一个理想的性状。为了鉴定调控[作物名称]种皮颜色的数量性状基因座（QTL），我们使用了一个重组自交系（RIL）群体，该群体由黄色种子的标准快速循环（RcBr）自交系09A001与深褐色种子的结球白菜自交系08A061杂交获得。通过两种表型评分方法，我们共检测到9个QTL，分布在4条染色体（Chrs.）A03、A06、A08和A09上，这些QTL解释了种子颜色表型变异的3.17%至55.73%。为了验证RIL群体中鉴定出的QTL的效应，选择了携带来自08A061的候选QTL区域染色体片段的染色体片段代换系（CSSL），并成功验证了两个共定位的主要QTL，[QTL名称1]和[QTL名称2]，以及一个次要QTL，[QTL名称3]。位于A03染色体上的已验证QTL似乎是[作物名称]种皮颜色的一个新基因座。这些发现提供了更多的见解，将有助于解释[作物名称]种皮颜色性状背后的复杂遗传机制。
