Zhang Yinghuan, Qin Yao, Li Dongxiao, Wang Wei, Gao Xu, Hao Chunming, Feng Hui, Wang Yugang, Li Tianlai
College of Horticulture, Shenyang Agricultural University, Shenyang, 110866, People's Republic of China.
Key Laboratory of Protected Horticulture, Ministry of Education, Shenyang Agricultural University, Shenyang, 110866, People's Republic of China.
Theor Appl Genet. 2023 Jan;136(1):11. doi: 10.1007/s00122-023-04287-0. Epub 2023 Jan 20.
A novel BrSCC1 gene for seed coat color was fine mapped within a 41.1-kb interval on chromosome A03 in Brassica rapa and functionally validated by ectopic expression analysis. Yellow seed is a valuable breeding trait that can be potentiality applied for improving seed quality and oil productivity in oilseed Brassica crops. However, only few genes for yellow seed have been identified in B. rapa. We previously identified a minor quantitative trait locus (QTL), qSC3.1, for seed coat color on chromosome A03 in B. rapa. In order to isolate the seed coat color gene, a brown-seeded chromosome segment substitution line, CSSL-38, harboring the qSC3.1, was selected and crossed with the yellow-seeded recurrent parent, a rapid cycling inbred line of B. rapa (RcBr), to construct the secondary F population. Metabolite identification suggested that seed coat coloration in CSSL-38 was independent of proanthocyanidins (PAs) accumulation. Genetic analysis revealed that yellow seed was controlled by a single recessive gene, Seed Coat Color 1 (BrSCC1). Utilizing bulked segregant analysis (BSA)-seq and secondary F and F recombinants analysis, BrSCC1 was fine mapped within a 41.1-kb interval. By integrating gene expression profiling, genome sequence comparison, metabolite analysis, and functional validation through ectopic expression in Arabidopsis, the BraA03g040800.3C gene was confirmed to be BrSCC1, which positively correlated with the seed coat coloration. Our study provides a novel gene resource for the genetic improvement of yellow seeds in oilseed B. rapa.
一个控制种皮颜色的新基因BrSCC1在白菜型油菜A03染色体上一个41.1 kb的区间内被精细定位，并通过异位表达分析进行了功能验证。黄色种子是一种有价值的育种性状，可潜在地用于提高油菜作物的种子品质和油生产率。然而，在白菜型油菜中仅鉴定出少数几个控制黄色种子的基因。我们之前在白菜型油菜A03染色体上鉴定了一个控制种皮颜色的微效数量性状位点（QTL）qSC3.1。为了分离种皮颜色基因，选择了携带qSC3.1的褐色种子染色体片段代换系CSSL-38，并与黄色种子轮回亲本——白菜型油菜快速循环自交系（RcBr）杂交，构建次级F群体。代谢物鉴定表明，CSSL-38的种皮颜色与原花青素（PAs）积累无关。遗传分析表明，黄色种子由一个单隐性基因种皮颜色1（BrSCC1）控制。利用混合分组分析法（BSA）-测序以及次级F和F重组体分析，将BrSCC1精细定位在一个41.1 kb的区间内。通过整合基因表达谱、基因组序列比较、代谢物分析以及在拟南芥中的异位表达功能验证，确认BraA03g040800.3C基因就是BrSCC1，它与种皮颜色呈正相关。我们的研究为油菜籽用白菜型油菜黄色种子的遗传改良提供了一种新的基因资源。
