体外和体内KAT复合物对赖氨酸乙酰化的测量与分析

Measurement and Analysis of Lysine Acetylation by KAT Complexes In Vitro and In Vivo.

作者信息

Lashgari Anahita, Lambert Jean-Philippe, Côté Jacques

机构信息

St. Patrick Research Group in Basic Oncology, Quebec City, QC, Canada.

Laval University Cancer Research Center, Quebec City, QC, Canada.

出版信息

Methods Mol Biol. 2019;1983:57-77. doi: 10.1007/978-1-4939-9434-2_5.

DOI:10.1007/978-1-4939-9434-2_5

PMID:31087293

Abstract

The acetylation of the ε-amine of lysine residues has significant impacts on the cellular functions of proteins. Through the combination of unbiased and targeted analysis of acetylated proteins, biological insights on lysine acetylation are now routinely generated. To help in this endeavor, we describe detailed protocols for the identification of acetylated lysine residues and the preparation of multiple reagents for the characterization of these sites in order to obtain functional insights on this widespread modification.

摘要

赖氨酸残基的ε-氨基乙酰化对蛋白质的细胞功能有重大影响。通过对乙酰化蛋白质进行无偏分析和靶向分析相结合，现在可以常规地获得关于赖氨酸乙酰化的生物学见解。为了助力这一工作，我们描述了用于鉴定乙酰化赖氨酸残基的详细方案以及用于表征这些位点的多种试剂的制备方法，以便获得关于这种广泛修饰的功能见解。