Liu Hongyan, Zhu Shu, Liu Qian, Zhang Yongqing
Teaching Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan, China.
Basic Medicine, Shandong University of Traditional Chinese Medicine, Jinan, China.
Biomed Chromatogr. 2019 Oct;33(10):e4583. doi: 10.1002/bmc.4583. Epub 2019 Jul 30.
Honeysuckle (Lonicera japonica flos) is a well-known agent of edible and medicinal value in China and its antioxidative activity makes a major contribution to its dual use. However, the compounds responsible for its antioxidative activity are still unknown. In this study, 10 batches of honeysuckle were collected from different origins in China. The fingerprints were established by HPLC technique to investigate the compounds and a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity assay was carried out to evaluate their antioxidant activity. partial least squares regression analysis was applied to set up the regression equation between DPPH radical scavenging activity and average peak area of common peaks of fingerprints. The results showed that peaks 10 (isochlorogenic acid B), 12 (isochlorogenic acid C), 11 (isochlorogenic acid A) and 9 (cynaroside) in the fingerprints were closely related to the antioxidant activity of 50% methanol extracts of honeysuckle. This study successfully established the spectrum-effect relationship between HPLC fingerprints and DPPH radical scavenging activity and provided a general model for exploring active components with a combination of chromatography and efficacy.
金银花（忍冬花）在中国是一种具有食用和药用价值的知名植物，其抗氧化活性对其双重用途起着重要作用。然而，负责其抗氧化活性的化合物仍然未知。在本研究中，从中国不同产地收集了10批金银花。采用高效液相色谱（HPLC）技术建立指纹图谱以研究化合物，并进行1,1-二苯基-2-苦基肼（DPPH）自由基清除活性测定以评估其抗氧化活性。应用偏最小二乘回归分析建立DPPH自由基清除活性与指纹图谱共有峰平均峰面积之间的回归方程。结果表明，指纹图谱中的峰10（异绿原酸B）、峰12（异绿原酸C）、峰11（异绿原酸A）和峰9（木犀草苷）与金银花50%甲醇提取物的抗氧化活性密切相关。本研究成功建立了HPLC指纹图谱与DPPH自由基清除活性之间的谱效关系，为结合色谱法和药效学探索活性成分提供了通用模型。
