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基于 HPLC 分析和抗氧化活性的西南产金银花质量评价。

Quality Evaluation of Lonicerae Flos Produced in Southwest China Based on HPLC Analysis and Antioxidant Activity.

机构信息

Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China.

Chongqing Institute for Food and Drug Control, Chongqing 401121, China.

出版信息

Molecules. 2024 May 29;29(11):2560. doi: 10.3390/molecules29112560.

DOI:10.3390/molecules29112560
PMID:38893434
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11173438/
Abstract

, the main source of traditional Chinese medicine Lonicerae Flos, is extensively cultivated in Southwest China. However, the quality of produced in this region significantly varies due to its wide distribution and various cultivation breeds. Herein, 50 Lonicerae Flos samples derived from different breeds of cultivated in Southwest China were collected for quality evaluation. Six organic acids and three saponin compounds were quantitatively analyzed using HPLC. Furthermore, the antioxidant activity of a portion of samples was conducted with 2,2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging experiments. According to the quantitative results, all samples met the quality standards outlined in the Chinese Pharmacopoeia. The samples from Guizhou, whether derived from unopened or open wild-type breeds, exhibited high quality, while the wild-type samples showed relatively significant fluctuation in quality. The samples from Chongqing and Hunan demonstrated similar quality, whereas those from Sichuan exhibited relatively lower quality. These samples demonstrated significant abilities in clearing ABTS and DPPH radicals. The relationship between HPLC chromatograms and antioxidant activity, as elucidated by multivariate analysis, indicated that chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, and isochlorogenic acid C are active components and can serve as Q-markers for quality evaluation.

摘要

金银花，作为中药的主要来源，在中国西南地区广泛种植。然而，由于其广泛的分布和各种种植品种，该地区生产的金银花质量差异很大。在此，我们收集了来自中国西南地区不同品种金银花的 50 个样本，用于质量评估。采用 HPLC 对 6 种有机酸和 3 种皂苷化合物进行定量分析。此外，我们还对部分样本的抗氧化活性进行了 2,2'-偶氮双-(3-乙基苯并噻唑啉-6-磺酸) (ABTS) 和 1,1-二苯基-2-苦基肼 (DPPH) 自由基清除实验。根据定量结果，所有样本均符合《中国药典》规定的质量标准。来自贵州的样本，无论是来自未开放还是开放的野生型品种，均表现出较高的质量，而野生型样本的质量波动相对较大。来自重庆和湖南的样本质量相似，而来自四川的样本质量相对较低。这些样本在清除 ABTS 和 DPPH 自由基方面表现出显著的能力。通过多元分析阐明的 HPLC 图谱与抗氧化活性之间的关系表明，绿原酸、异绿原酸 A、异绿原酸 B 和异绿原酸 C 是活性成分，可以作为质量评价的 Q-标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e2f/11173438/6716a10596c2/molecules-29-02560-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e2f/11173438/de933b6d19fe/molecules-29-02560-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e2f/11173438/effe97f1050a/molecules-29-02560-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e2f/11173438/b606b83f2333/molecules-29-02560-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e2f/11173438/fc2b800c78c8/molecules-29-02560-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e2f/11173438/c5cf40af2186/molecules-29-02560-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e2f/11173438/37849e66700d/molecules-29-02560-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e2f/11173438/f4a9517e2725/molecules-29-02560-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e2f/11173438/6716a10596c2/molecules-29-02560-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e2f/11173438/de933b6d19fe/molecules-29-02560-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e2f/11173438/effe97f1050a/molecules-29-02560-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e2f/11173438/b606b83f2333/molecules-29-02560-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e2f/11173438/fc2b800c78c8/molecules-29-02560-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e2f/11173438/c5cf40af2186/molecules-29-02560-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e2f/11173438/37849e66700d/molecules-29-02560-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e2f/11173438/f4a9517e2725/molecules-29-02560-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e2f/11173438/6716a10596c2/molecules-29-02560-g008.jpg

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