Yu Xue, Sun Hong-Wei, Li Wei-Wei, Qi Guo-Ping, Ma Juan, Cheng Yong-Zhi, Lü Xin-Tao
School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China.
Sewage Treatment Industry Technical Center of Gansu Province, Lanzhou 730070, China.
Huan Jing Ke Xue. 2019 Mar 8;40(3):1426-1430. doi: 10.13227/j.hjkx.201808261.
A sequencing batch reactor (SBR) was operated in this study to investigate the effect of temperature on the kinetics of activity among nitrite oxidizing bacteria. At the beginning of the experiment, the NO-N concentration in the influent was changed to enrich . Then, the sludge with enriched was employed to determine the variation of the specific nitrite oxidation rate (SNiOR) during the nitrite oxidation process in batch tests. Metagenomics species annotation and abundance analysis showed that accounted for 40.3% of the total bacterial population. The variation of SNiOR in the nitrite oxidation process was investigated under different NO-N concentrations. The effect of temperature on the kinetics of was investigated using the Monod model. Furthermore, the kinetics model of the effect of temperature on activity was fitted for statistical analysis. The results showed that SNiOR reached its maximum at 30℃, which was 1.31 g·(g·d). Statistical analysis showed that the Monod equation could describe the effect of substrate concentration on activity under different temperature conditions. Calculating the temperature coefficient () in different temperature intervals based on the Phelps equation, showed that when the system temperature is lower than 25℃ or higher than 30℃, the reaction rate is more sensitive to temperature changes.
本研究运行了一个序批式反应器(SBR),以研究温度对亚硝酸盐氧化细菌活性动力学的影响。在实验开始时,改变进水的NO-N浓度以进行富集。然后,使用富集后的污泥在批次试验中确定亚硝酸盐氧化过程中特定亚硝酸盐氧化速率(SNiOR)的变化。宏基因组学物种注释和丰度分析表明,[具体细菌名称]占细菌总数的40.3%。在不同的NO-N浓度下研究了亚硝酸盐氧化过程中SNiOR的变化。使用莫诺德模型研究了温度对[具体细菌名称]动力学的影响。此外,对温度对[具体细菌名称]活性影响的动力学模型进行拟合以进行统计分析。结果表明,SNiOR在30℃时达到最大值,为1.31 g·(g·d)。统计分析表明,莫诺德方程可以描述不同温度条件下底物浓度对[具体细菌名称]活性的影响。根据费尔普斯方程计算不同温度区间的温度系数(),结果表明当系统温度低于25℃或高于30℃时,反应速率对温度变化更敏感。