HydroSciences Montpellier (HSM), Institut de Recherche pour le Développement (IRD), CNRS, Université Montpellier, Montpellier, France.
Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand.
Parasit Vectors. 2019 May 14;12(1):223. doi: 10.1186/s13071-019-3494-8.
The Barbirostris Complex comprises six formally described species that cannot be differentiated based on morphology alone. Out of these six species, two have been reported as putative malaria vectors, An. campestris and An. wejchoochotei. Five species are present in Thailand, An. barbirostris, An. campestris, An. dissidens, An. saeungae and An. wejchoochotei, while An. vanderwulpi occurs in Indonesia. As these species cannot be accurately differentiated by morphological characters, there is a crucial lack of information on their bionomics and role in the transmission of malaria and filariasis agents.
For differentiating the six species, an allele-specific amplification (AS-PCR) based on the second internal transcribed spacer (ITS2) sequence was developed. From 862 mosquitoes in the Barbirostris Complex collected in 23 provinces throughout Thailand, the AS-PCR was able to identify five species and its validation was undertaken on 185 specimens.
This multiplex-PCR assay is potentially able to definitely identify all six species of the Barbirostris Complex and was validated on five species present in Thailand.
巴比罗龙复合群由六个形态上无法区分的正式描述种组成。这六个种中有两种被报道为潜在的疟疾媒介，即按蚊 campestris 和按蚊 wejchoochotei。五种种存在于泰国，即按蚊 barbirostris、按蚊 campestris、按蚊 dissidens、按蚊 saeungae 和按蚊 wejchoochotei，而按蚊 vanderwulpi 则存在于印度尼西亚。由于这些种不能通过形态特征准确区分，因此它们的生物学和在疟疾和丝虫病媒介传播中的作用的信息严重缺乏。
为了区分这六个种，基于第二个内部转录间隔区（ITS2）序列开发了一种基于等位基因特异性扩增（AS-PCR）的方法。从在泰国 23 个省采集的 862 只巴比罗龙复合群蚊子中，AS-PCR 能够识别出 5 个种，并在 185 个标本上进行了验证。
这种多重 PCR 检测方法可能能够明确识别巴比罗龙复合群的所有六个种，并在泰国存在的五个种上进行了验证。
