来自印度的野生动物狂犬病病毒分离株的分子流行病学分析。
Molecular epidemiological analysis of wild animal rabies isolates from India.
作者信息
Reddy Gundallhalli Bayyappa Manjunatha, Singh Rajendra, Singh Karam Pal, Sharma Anil Kumar, Vineetha Sobharani, Saminathan Mani, Sajjanar Basavaraj
机构信息
Department of Veterinary Pathology, ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka, India.
Department of Veterinary Pathology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, India.
出版信息
Vet World. 2019;12(3):352-357. doi: 10.14202/vetworld.2019.352-357. Epub 2019 Mar 4.
AIM
This study was conducted to know the genetic variability of rabies viruses (RVs) from wild animals in India.
MATERIALS AND METHODS
A total of 20 rabies suspected brain samples of wild animals from different states of India were included in the study. The samples were subjected for direct fluorescent antibody test (dFAT), reverse transcription polymerase chain reaction (RT-PCR), and quantitative reverse transcriptase real-time PCR (RT-qPCR). The phylogenetic analysis of partial nucleoprotein gene sequences was performed.
RESULTS
Of 20 samples, 11, 10, and 12 cases were found positive by dFAT, RT-PCR, and RT-qPCR, respectively. Phylogenetic analysis showed that all Indian wild RVs isolates belonged to classical genotype 1 of Lyssavirus and were closely related to Arctic/Arctic-like single cluster indicating the possibility of a spillover of rabies among different species.
CONCLUSION
The results indicated the circulation of similar RVs in sylvatic and urban cycles in India. However, understanding the role of wild animals as reservoir host needs to be studied in India.
目的
本研究旨在了解印度野生动物狂犬病病毒（RVs）的遗传变异性。
材料与方法
本研究纳入了来自印度不同邦的共20份野生动物狂犬病疑似脑样本。对样本进行直接荧光抗体试验（dFAT）、逆转录聚合酶链反应（RT-PCR）和定量逆转录实时聚合酶链反应（RT-qPCR）。对部分核蛋白基因序列进行系统发育分析。
结果
在20份样本中，分别有11例、10例和12例通过dFAT、RT-PCR和RT-qPCR检测呈阳性。系统发育分析表明，所有印度野生RVs分离株均属于狂犬病病毒的经典基因型1，且与北极/类北极单簇密切相关，这表明狂犬病在不同物种间存在溢出传播的可能性。
结论
结果表明，类似的RVs在印度的野生动物和城市传播循环中存在。然而，在印度，需要研究野生动物作为储存宿主的作用。
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