Farjami Fatemeh, Fasihi Farshid, Alimohammadi Forough, Moradi Seyed Esmaeil
Pharmaceutical Sciences Research Centre, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Chemistry, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran.
Iran J Pharm Res. 2019 Winter;18(1):91-101.
In this manuscript, the electrocatalytic oxidation of doxepin (DOX) was studied at a carbon ionic liquid electrode, fabricated using graphite, and the ionic liquid 1-octylpyridinium hexaflourophosphate (OPFP). The surface of the proposed electrode was characterized by scanning electron microscopy. Differential pulse voltammetry was applied as an analytical technique for quantification of sub-micromolar concentration of doxepin. Various parameters were optimized for practical application. Under the optimal conditions, the proposed electrode exhibited interesting sensitivity toward determination of doxepin compared to the other conventional electrodes and the anodic peak current versus doxepin concentration was linear in the ranges of 0.05-24 µM. The detection limit of 21 nM was achieved. Cyclic voltammetry (CV) was also applied to acquire information about the reaction mechanism and calculating the kinetic parameters. The electroxidation process was irreversible and revealed adsorption controlled behavior. The method was successfully applied for determination of doxepin content in pharmaceuticals and blood serum samples.
在本手稿中，研究了在由石墨和离子液体1-辛基吡啶六氟磷酸盐（OPFP）制成的碳离子液体电极上多塞平（DOX）的电催化氧化。通过扫描电子显微镜对所提出电极的表面进行了表征。采用差分脉冲伏安法作为分析技术来定量亚微摩尔浓度的多塞平。对各种参数进行了优化以用于实际应用。在最佳条件下，与其他传统电极相比，所提出的电极对多塞平的测定表现出有趣的灵敏度，并且阳极峰电流与多塞平浓度在0.05 - 24 μM范围内呈线性关系。实现了21 nM的检测限。还应用循环伏安法（CV）来获取有关反应机理的信息并计算动力学参数。电氧化过程是不可逆的，并显示出吸附控制行为。该方法成功应用于药物和血清样品中多塞平含量的测定。
