Misni Norashiqin, Mohamed Nor Zurainee, Ahmad Rohani
Department of Parasitology, Faculty of Medicine, University Malaya, Kuala Lumpur, Malaysia.
Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor.
Iran J Pharm Res. 2019 Winter;18(1):198-209.
Essential oil of Citrus family plant is known to have repellent effect against mosquito. Unfortunately, due to its high volatility effect, its repellency effect was compromised. The incorporation of essential oil in a microencapsulation formulation has been shown to help improve the stability and potency of the repellent. In this study, peel oil (CGPO) was encapsulated by using the interfacial precipitation chemistry technique. The microencapsulated CGPO was then formulated into lotion form to produce topical repellent formulation. This study includes the characterization of microcapsules with regards to the morphology, size distribution, zeta potential, Fourier Transmission Infrared spectrophotometer (FTIR), and Thermogravity analysis (TGA). The effectiveness of the microencapsulated CGPO-lotion formulation against mosquitoes was evaluated in the laboratory setting. Results indicated that CGPO have been successfully encapsulated with 6.5 µm in diameter and zeta potential values, -47.9 mV. The FTIR analysis spectrum indicated the presence of interaction between the wall materials in microcapsules. The TGA analysis demonstrated that microencapsulation improved the thermal stability of CGPO. Repellency assay revealed that microencapsulated CGPO- based formulation possessed excellent effect compared with pure CGPO. In conclusion, CGPO was successfully encapsulated and the microencapsulation aid to improve the repellency effect of CGPO against mosquito bites.
已知柑橘科植物的精油对蚊子有驱避作用。不幸的是，由于其高挥发性，其驱避效果受到影响。已证明将精油纳入微囊化制剂有助于提高驱避剂的稳定性和效力。在本研究中，采用界面沉淀化学技术对果皮油（CGPO）进行微囊化。然后将微囊化的CGPO制成乳液形式，以生产局部驱避剂制剂。本研究包括对微胶囊的形态、尺寸分布、zeta电位、傅里叶变换红外光谱仪（FTIR）和热重分析（TGA）进行表征。在实验室环境中评估了微囊化CGPO乳液制剂对蚊子的有效性。结果表明，CGPO已成功微囊化，直径为6.5 µm，zeta电位值为-47.9 mV。FTIR分析光谱表明微胶囊中壁材之间存在相互作用。TGA分析表明微囊化提高了CGPO的热稳定性。驱避试验表明，与纯CGPO相比，基于微囊化CGPO的制剂具有优异的效果。总之，CGPO成功微囊化，微囊化有助于提高CGPO对蚊虫叮咬的驱避效果。
