Eftekhari Mahdieh, Shams Ardekani Mohammad Reza, Amin Mohsen, Attar Farideh, Akbarzadeh Tahmineh, Safavi Maliheh, Karimpour-Razkenari Elahe, Amini Mohsen, Isman Murray, Khanavi Mahnaz
Department of Pharmacognosy, Faculty of Pharmacy and Persian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Pharm Res. 2019 Winter;18(1):412-421.
is an aromatic plant traditionally used for treatment of infections and gastrointestinal diseases. In the present study, the volatile oil of the plant was obtained by hydrodistillation and analyzed by GC-MS. In addition, antibacterial and anti- activities of this essential oil were determined using disc diffusion and agar dilution methods, respectively. Insecticidal activity was assessed through topical and fumigation application of the essential oil to cabbage looper larvae. Acetylcholinesterase (AChE) inhibition by the essential oil was examined using Ellman's method. Furthermore, its cytotoxic potential against three different cancer cell lines was assessed using the MTT assay. The phenolic monoterpenoids, thymol (38.79%), and carvacrol (36.30%) were identified as major constituents of the essential oil. We observed significant antibacterial activity of the essential oil against (MIC=20.4 µg /mL) as well as other tested bacteria, except for . essential oil showed significant toxicity to cabbage looper larvae with LD value of 52.1 µg /larva following topical and fumigant administration. essential oil was considerably inhibitory to acetylcholinesterase activity (IC50 = 0.117 µg/mL). Cytotoxic assay of the volatile oil resulted in IC50 = 0.065, 0.104, and 0.141 μg/mL for MCF-7, T47D and MDA- MB-231 cell lines, respectively. According to our data, this species with high concentrations of thymol and carvacrol could be considered as a natural source for pharmaceutical products.
是一种传统上用于治疗感染和胃肠道疾病的芳香植物。在本研究中，通过水蒸馏法获得该植物的挥发油，并采用气相色谱 - 质谱联用（GC - MS）进行分析。此外，分别使用纸片扩散法和琼脂稀释法测定了这种精油的抗菌和抗[此处原文缺失部分内容]活性。通过将精油局部涂抹和熏蒸处理甘蓝夜蛾幼虫来评估其杀虫活性。使用埃尔曼方法检测精油对乙酰胆碱酯酶（AChE）的抑制作用。此外，使用MTT法评估其对三种不同癌细胞系的细胞毒性潜力。酚类单萜化合物百里香酚（38.79%）和香芹酚（36.30%）被鉴定为该精油的主要成分。我们观察到该精油对[此处原文缺失部分内容]（MIC = 20.4 µg /mL）以及其他受试细菌具有显著的抗菌活性，但对[此处原文缺失部分内容]除外。该精油对甘蓝夜蛾幼虫表现出显著毒性，局部涂抹和熏蒸给药后的LD值为52.1 µg /幼虫。该精油对乙酰胆碱酯酶活性具有相当大的抑制作用（IC50 = 0.117 µg/mL）。挥发油的细胞毒性测定结果显示，对MCF - 7、T47D和MDA - MB - 231细胞系的IC50分别为0.065、0.104和0.141 μg/mL。根据我们的数据，这种富含百里香酚和香芹酚的植物可被视为药品的天然来源。
