Khoshbakht Tahereh, Karami Akbar, Tahmasebi Aminallah, Maggi Filippo
Department of Horticultural Science, School of Agriculture, Shiraz University, 71441-65186 Shiraz, Iran.
Department of Agriculture, Minab Higher Education Center, University of Hormozgan, 84156-83111 Bandar Abbas, Iran.
Antibiotics (Basel). 2020 Jul 14;9(7):409. doi: 10.3390/antibiotics9070409.
(Apiaceae) is an aromatic herb traditionally employed in the Persian medicine for the treatment of infectious and gastrointestinal disorders. In the present study, we analyzed the chemical composition of essential oils obtained from different Iranian populations and evaluated their efficacy on a panel of human pathogens ( and ), probiotic (), and phytopathogens (, , , and ). The gas chromatographic-mass spectrometry analysis put in evidence four main volatile constituents such as thymol (20.3-36.4%), carvacrol (18.8-33.1%), γ-terpinene (10.6-25.9%), and -cymene (9.5-17.3%), though with significant variability from an essential oil to another. Notably, the oils from the populations sited in Nourabad Mamasani and Dehdasht showed the highest amount of the phenolic monoterpenes thymol (36.4 and 35.2%, respectively) and carvacrol (33.1 and 30.6%, respectively). The antibacterial activity of essential oils was assessed by the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) methods, showing high activity for the samples from Nourabad Mamasani and Dehdasht populations exhibiting high level of the above phenolics. The obtained MIC and MBC values (mg/ml) were in the ranges 0.0625-2 mg/ml and 1-16 mg/ml, respectively. Noteworthy, in some cases, the antibacterial activity of essential oils was higher than that of chloramphenicol used as positive control. The average MBCs displayed by the samples showed that had the highest sensitivity to the essential oils. Based on these results, our work shed light on selected populations deserving proper breeding and cultivation strategies in order to warrantee production of bioactive essential oils to be used at pharmaceutical and agricultural level to combat several pathogens.
伞形科是一种芳香草本植物，传统上用于波斯医学中治疗感染性疾病和胃肠道疾病。在本研究中，我们分析了从不同伊朗种群获得的精油的化学成分，并评估了它们对一组人类病原体（和）、益生菌（）和植物病原体（、、和）的功效。气相色谱 - 质谱分析表明有四种主要挥发性成分，即百里香酚（20.3 - 36.4%）、香芹酚（18.8 - 33.1%）、γ-萜品烯（10.6 - 25.9%）和对异丙基甲苯（9.5 - 17.3%），不过不同精油之间存在显著差异。值得注意的是，位于努拉巴德马马萨尼和德赫达什特的种群所产的精油中酚类单萜百里香酚（分别为36.4%和35.2%）和香芹酚（分别为33.1%和30.6%）的含量最高。通过最低抑菌浓度（MIC）和最低杀菌浓度（MBC）方法评估了精油的抗菌活性，结果显示来自努拉巴德马马萨尼和德赫达什特种群且上述酚类含量高的样品具有高活性。所获得的MIC和MBC值（mg/ml）分别在0.0625 - 2 mg/ml和1 - 16 mg/ml范围内。值得注意的是，在某些情况下，精油的抗菌活性高于用作阳性对照的氯霉素。样品显示的平均MBC表明对精油最敏感。基于这些结果，我们的工作揭示了一些值得采用适当育种和栽培策略的选定种群，以确保生产出可用于制药和农业层面抗击多种病原体的生物活性精油。
