Anti Compounds from Vent. through Urease Inhibitory and Studies.

Vent. has been used by indigenous people of southwest Iran for treating peptic ulcers and gastrointestinal infections. This study aimed to investigate the antibacterial activity of extract and fractions and to analyze the bioactive components of the fractions. Total plant extract and different fractions of Vent. were prepared. Antibacterial activities were evaluated against the clinical strain of and standard strains of , , and using agar dilution and disc diffusion methods. Phytochemical analysis of the fractions was performed using silica gel chromatography and 1D and 2D NMR spectroscopy. Moreover, the urease inhibitory effects of the isolated compounds were assessed and . Three novel kaempferol derivatives and two thymol derivatives were isolated from aerial parts, and the structures were determined by comparison with published data. The -hexane fraction was found to exert the most significant anti activity with the minimum inhibitory concentration of 50 µg/mL. All fractions demonstrated antibacterial activity toward . urease inhibition assay showed that stigmasterol, tiliroside, and carvacrol were found to be the most potent enzyme inhibitors in the isolated compounds. Molecular interactions of the compounds with the active site of urease were supported by the molecular docking analysis. Novel bioactive compounds in were described in this study. The antibacterial effects suggested the potential use of the compounds in pharmaceutical formulations inconsistent with the traditional use of the plant in the treatment of gastrointestinal infections.