School of Medical Technology, Xuzhou Medical University, Xuzhou Jiangsu 221004, China.
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou Jiangsu 215163, China.
Dis Markers. 2019 Apr 4;2019:5232780. doi: 10.1155/2019/5232780. eCollection 2019.
Combination of multiple biomarkers was an effective strategy to improve sensitivity in cancer diagnosis and screening. However, the performance of the combination of methylated and for detection of colorectal cancer (CRC) has yet to be reported.
A new qPCR-based assay combining the detection of methylated and was used. Methylation statuses of and were examined in 19 sets of cancer tissues and paired adjacent tissues and further evaluated with 225 serum samples, including 111 CRC patients and 114 no evidence of disease individuals.
and methylation levels were higher in 94.7% and 100.0% of cancer tissues than in their paired adjacent tissues. The sensitivities for detecting CRC by methylation alone and methylation alone were 73.0% (95% CI: 63.6-80.8%) and 71.2% (95% CI: 61.8-79.2%), respectively, with the same specificity of 95.6% (95% CI: 89.6-98.4%). However, when methylation was combined with methylation to detect CRC, the sensitivity was improved to 86.5% (95% CI: 78.4-92.0%) with a specificity of 92.1% (95% CI: 85.1-96.1%).
The combination of methylated and detection in serum has the potential to be a noninvasive strategy for CRC screening.
联合多种生物标志物是提高癌症诊断和筛查灵敏度的有效策略。然而，联合甲基化和用于检测结直肠癌（CRC）的性能尚未见报道。
采用一种新的基于 qPCR 的联合检测甲基化和的方法。检测了 19 对癌组织及其配对的癌旁组织中的甲基化状态，并进一步用 225 份血清样本进行了评估，包括 111 例 CRC 患者和 114 例无疾病证据的个体。
和的甲基化水平在 94.7%和 100.0%的癌组织中高于其配对的癌旁组织。单独检测甲基化和单独检测的敏感性分别为 73.0%（95%CI：63.6-80.8%）和 71.2%（95%CI：61.8-79.2%），特异性均为 95.6%（95%CI：89.6-98.4%）。然而，当联合检测甲基化和甲基化来检测 CRC 时，敏感性提高到 86.5%（95%CI：78.4-92.0%），特异性为 92.1%（95%CI：85.1-96.1%）。
血清中联合检测甲基化和的方法具有成为 CRC 筛查的非侵入性策略的潜力。
