Lysenko Evgeny A, Popov Daniil V, Vepkhvadze Tatiana F, Sharova Anna P, Vinogradova Olga L
Laboratory of Exercise Physiology, SSC RF Institute of Biomedical Problems RAS, Moscow, Russia.
Faculty of Fundamental Medicine, M.V. Lomonosov Moscow State University, Moscow, Russia.
Physiol Rep. 2019 May;7(9):e14100. doi: 10.14814/phy2.14100.
We examined signaling responses in the skeletal muscle of strength athletes after strength exercises under high and moderate load. Eight trained male powerlifters were recruited. The volunteers performed four sets of leg presses to volitional fatigue using a moderate load (65% 1-repetition maximum [1RM]) for one leg, and a high load (85% 1RM) for the contralateral leg. The work volume performed by the leg moving a moderate load was higher than that of the contralateral leg moving a high load. Biopsy of the m. vastus lateralis was performed before, and at 1, 5, and 10 h after, cessation of exercise. Phosphorylation of p70S6k , 4E-BP1 , and ACC increased after moderate load exercises, whereas phosphorylation of ERK1/2 increased, and that of eEF2 decreased, after high load exercises. Exercise under a moderate load and a high work volume activated mTORC1-dependent signaling in trained skeletal muscle, whereas exercise under a high load but lower work volume activated the MEK-ERK1/2 signaling cascade and eEF2.
我们研究了力量型运动员在高强度和中等强度负荷力量训练后骨骼肌中的信号反应。招募了8名训练有素的男性举重运动员。志愿者们一条腿使用中等负荷（65%的一次重复最大值[1RM]）进行四组腿举至意志性疲劳，对侧腿使用高负荷（85%的1RM）进行同样的操作。移动中等负荷的腿所完成的工作量高于移动高负荷的对侧腿。在运动停止前、运动停止后1小时、5小时和10小时对股外侧肌进行活检。中等负荷运动后，p70S6k、4E-BP1和ACC的磷酸化增加，而高负荷运动后，ERK1/2的磷酸化增加，eEF2的磷酸化减少。中等负荷且高工作量的运动激活了训练有素的骨骼肌中mTORC1依赖性信号传导，而高负荷但低工作量的运动激活了MEK-ERK1/2信号级联和eEF2。
