Bao Hong-Song, Hou Jing-Yu, Hu He-Jia, Li Yue-Ting, Zheng Lin, Huang Yong, Wang Guang-Cheng, Zhou Meng, Lan Yan-Yu, Gong Zi-Peng
School of Pharmacy, Guizhou Medical University, Provincial Key Laboratory of Pharmaceutics in Guizhou,State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine Guiyang 550004, China Department of Pharmacy, Guizhou Orthopedics Hospital Guiyang 550002, China.
School of Pharmacy, Guizhou Medical University, Provincial Key Laboratory of Pharmaceutics in Guizhou,State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Provincial Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine Guiyang 550004, China.
Zhongguo Zhong Yao Za Zhi. 2019 Apr;44(7):1475-1484. doi: 10.19540/j.cnki.cjcmm.20190116.007.
To determine the plasma protein binding rate of the nine compounds in Inula cappa extraction by the method of equilibrium dialysis. The proteins in plasma samples were precipitated by methanol, and the ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) was developed for determination of the concentrations of the nine active compounds, namely chlorogenic acid, scopolin, neochlorogenic acid, cryptochlorogenic acid, 1,3-O-dicaffeoylquinic acid, galuteolin, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid, with the internal standard of puerarin. We found that all components have a good linearity(r≥0.999), and accuracy, precision, extraction recovery and stability conformed to the requirements of determination, without endogenous compounds disturbing within the range of optimum concentration. This suggested that the method was stable and reliable, and could be used for the determination of the plasma protein binding rates of the nine active compounds in rat and human plasma of I. cappa. The plasma protein binding rates of the nine active compounds in rat and human plasma respectively were(41.07±0.046)%-(94.95±0.008)%, and(37.66±0.043)%-(97.46±0.013)%. According to the results, there were differences in the plasma protein binding rates of the nine compounds in I. cappa extraction between rat and human.
采用平衡透析法测定九里香提取物中9种化合物的血浆蛋白结合率。用甲醇沉淀血浆样品中的蛋白质，建立超高效液相色谱-串联质谱法（UPLC-MS/MS）测定9种活性成分，即绿原酸、东莨菪苷、新绿原酸、隐绿原酸、1,3-O-二咖啡酰奎宁酸、木犀草素、3,4-O-二咖啡酰奎宁酸、3,5-O-二咖啡酰奎宁酸、4,5-O-二咖啡酰奎宁酸的浓度，以葛根素为内标。结果表明，各成分线性关系良好（r≥0.999），准确度、精密度、提取回收率和稳定性均符合测定要求，在最佳浓度范围内无内源性化合物干扰。说明该方法稳定可靠，可用于测定九里香提取物中9种活性成分在大鼠和人血浆中的血浆蛋白结合率。9种活性成分在大鼠和人血浆中的血浆蛋白结合率分别为（41.07±0.046）% - （94.95±0.008）%和（37.66±0.043）% -（97.46±0.013）%。结果显示，九里香提取物中9种化合物在大鼠和人血浆中的血浆蛋白结合率存在差异。
