Gong Zi-Peng, Li Mei, Hou Jing-Yu, Wu Lin-Lin, Chen Ting-Ting, Li Yue-Ting, Chen Si-Ying, Li Yong-Jun, Wang Ai-Min, Lan Yan-Yu, Wang Yong-Lin
Guizhou Provincial Key Laboratory of Pharmaceutics， Guizhou Medical University， Guiyang 550004， China.
Engineering Research Center for the Development and Application of Ethnic Medicine and Traditional Chinese Medicine， Ministry of Education， Guizhou Medical University， Guiyang 550004， China.
Zhongguo Zhong Yao Za Zhi. 2018 Feb;43(3):609-617. doi: 10.19540/j.cnki.cjcmm.20171211.003.
To investigate the absorptive characteristics of Inula cappa extract based on the rat everted intestinal sac method . Nine representative ingredients in I. cappa extract were selected as the study objects. An UPLC-MS/MS method was established to determine and detect their cumulative absorption amount for expounding the absorptive characteristics of ingredients in different intestinal sections. According to the results， the transport mechanism of 8 compounds showed passive diffusion by the reverted gut sac method. And scopolin was actively transported in the intestine. The best absorption site of chlorogenic acid was duodenum. The best absorption site of cryptochlorogenic acid， 1，3--dicaffeoylquinic acid， luteolin-7-glucoside and 3，4--dicaffeoylquinic acid were jejunum. The best absorption site of neochlorogenic acid， scopolin， 4，5--dicaffeoylquinic acid and 3，5--dicaffeoylquinic acid was ileum. The absorption of all the compounds was affected by pH and bile. All of the nine ingredients in I. cappa extract could be absorbed in intestines， but with differences in the absorption rate， the best absorptive site and mechanism， indicating that the intestinal absorption of I. cappa extract was selective.
采用大鼠外翻肠囊法研究戴叶旋覆花提取物的吸收特性。选取戴叶旋覆花提取物中的9种代表性成分作为研究对象。建立超高效液相色谱-串联质谱（UPLC-MS/MS）法测定并检测其累积吸收量，以阐明不同肠段成分的吸收特性。结果显示，8种化合物的转运机制通过外翻肠囊法表现为被动扩散。东莨菪苷在肠道中为主动转运。绿原酸的最佳吸收部位是十二指肠。隐绿原酸、1,3-二咖啡酰奎宁酸、木犀草素-7-葡萄糖苷和3,4-二咖啡酰奎宁酸的最佳吸收部位是空肠。新绿原酸、东莨菪苷、4,5-二咖啡酰奎宁酸和3,5-二咖啡酰奎宁酸的最佳吸收部位是回肠。所有化合物的吸收均受pH值和胆汁的影响。戴叶旋覆花提取物中的9种成分均可在肠道吸收，但在吸收率、最佳吸收部位和机制方面存在差异，表明戴叶旋覆花提取物的肠道吸收具有选择性。
