Bone Res Lab, School of Dentistry of Ribeirao Preto, University of São Paulo, Ribeirao Preto, Brazil.
Regional Blood Center of Ribeirão Preto, University of São Paulo, Ribeirao Preto, Brazil.
J Cell Biochem. 2019 Oct;120(10):16723-16732. doi: 10.1002/jcb.28930. Epub 2019 May 15.
Topographical modifications of titanium (Ti) at the nanoscale level generate surfaces that regulate several signaling pathways and cellular functions, which may affect the process of osseointegration. Here, we investigated the participation of integrin αV in the osteogenic capacity of Ti with nanotopography. Machined titanium discs (untreated) were submitted to treatment with H SO /H O to produce the nanotopography (nanostructured). First, the greater osteogenic capacity of the nanotopography that increased osteoblast differentiation of mesenchymal stem cells compared with untreated topography was shown. Also, the nanostructured surface increased (regulation ≥ 1.9-fold) the gene expression of 6 integrins from a custom array plate utilized to evaluate the gene expression of 84 genes correlated with cell adhesion signaling pathway, including integrin αV, which is involved in osteoblast differentiation. By silencing integrin αV in MC3T3-E1 cells cultured on nanotopography, the impairment of osteoblast differentiation induced by this surface was observed. In conclusion, it was shown that nanotopography regulates the expression of several components of the cell adhesion signaling pathway and its higher osteogenic potential is, at least in part, due to its ability to upregulate the expression of integrin αV. Together with previous data that showed the participation of integrins α1, β1, and β3 in the nanotopography osseoinduction activity, we have uncovered the pivotal role of this family of membrane receptors in the osteogenic potential of this surface.
钛（Ti）的纳米级形貌修饰会产生调节多种信号通路和细胞功能的表面，这可能会影响骨整合过程。在这里，我们研究了整合素αV 在具有纳米形貌的 Ti 的成骨能力中的参与作用。机加工的钛盘（未经处理）经过 H SO /H O 处理以产生纳米形貌（纳米结构）。首先，与未经处理的形貌相比，纳米形貌增加了间充质干细胞的成骨分化，显示出更高的成骨能力。此外，纳米结构表面增加（调节≥1.9 倍）了定制阵列板上 6 种整合素的基因表达，该板用于评估与细胞黏附信号通路相关的 84 个基因的基因表达，包括参与成骨细胞分化的整合素αV。通过在纳米形貌上培养的 MC3T3-E1 细胞中沉默整合素αV，观察到该表面诱导的成骨细胞分化受损。总之，结果表明纳米形貌调节细胞黏附信号通路的几个组成部分的表达，其更高的成骨潜力至少部分归因于其上调整合素αV 表达的能力。结合之前表明整合素α1、β1 和β3 参与纳米形貌诱导成骨活性的数据，我们揭示了这个膜受体家族在该表面成骨潜力中的关键作用。
