Schneider René, Sampathkumar Arun, Persson Staffan
Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
School of Biosciences, University of Melbourne, Parkville, Victoria, Australia.
Curr Protoc Plant Biol. 2019 Jun;4(2):e20091. doi: 10.1002/cppb.20091. Epub 2019 May 15.
The cytoskeleton is key to many essential processes in a plant cell, e.g., growth, division, and defense. Contrary to what "skeleton" implies, the cytoskeleton is highly dynamic, and is able to re-organize itself continuously. The advent of live-cell microscopy and the development of genetically encoded fluorophores enabled detailed observation of the organization and dynamics of the cytoskeleton. Despite the biological importance of the cytoskeletal dynamics, quantitative analyses remain laborious endeavors that only a handful of research teams regularly conduct. With this protocol, we provide a standardized step-by-step guide to analyze the dynamics of microtubules. We provide example data and code for post-processing in Fiji that enables researchers to modify and adapt the routine to their needs. More such tools are needed to quantitatively assess the cytoskeleton and thus to better understand cell biology. © 2019 by John Wiley & Sons, Inc.
细胞骨架是植物细胞中许多重要过程的关键，例如生长、分裂和防御。与“骨架”所暗示的不同，细胞骨架具有高度的动态性，并且能够不断自我重新组织。活细胞显微镜技术的出现以及基因编码荧光团的发展，使得对细胞骨架的组织和动态进行详细观察成为可能。尽管细胞骨架动态在生物学上具有重要意义，但定量分析仍然是一项艰巨的工作，只有少数研究团队经常进行。通过本方案，我们提供了一个标准化的分步指南来分析微管的动态。我们提供了在 Fiji 中进行后处理的示例数据和代码，使研究人员能够根据自己的需求修改和调整该程序。需要更多这样的工具来定量评估细胞骨架，从而更好地理解细胞生物学。© 2019 约翰威立父子公司
