Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu, China.
Department of System Integration, Sparebanken Vest, Bergen, Norway.
Brief Bioinform. 2020 May 21;21(3):946-956. doi: 10.1093/bib/bbz045.
The completion of the rice genome sequence paved the way for rice functional genomics research. Additionally, the functional characterization of transcription factors is currently a popular and crucial objective among researchers. Transcription factors are one of the groups of proteins that bind to either enhancer or promoter regions of genes to regulate expression. On the basis of several typical examples of transcription factor analyses, we herein summarize selected research strategies and methods and introduce their advantages and disadvantages. This review may provide some theoretical and technical guidelines for future investigations of transcription factors, which may be helpful to develop new rice varieties with ideal traits.
水稻基因组序列的完成为水稻功能基因组学研究铺平了道路。此外，转录因子的功能表征目前是研究人员的一个热门和关键目标。转录因子是一类能够结合基因的增强子或启动子区域来调节表达的蛋白质。基于转录因子分析的几个典型例子，我们在这里总结了一些选择的研究策略和方法，并介绍了它们的优缺点。本综述可能为未来转录因子的研究提供一些理论和技术指导，有助于开发具有理想特性的新型水稻品种。
