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GH3细胞中形成的肌醇磷酸多种异构体的分离。

Separation of multiple isomers of inositol phosphates formed in GH3 cells.

作者信息

Dean N M, Moyer J D

出版信息

Biochem J. 1987 Mar 1;242(2):361-6. doi: 10.1042/bj2420361.

DOI:10.1042/bj2420361
PMID:3109388
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1147713/
Abstract

A high-performance-liquid-chromatography (h.p.l.c.) separation was developed, which resolves isomers of inositol monophosphate (IP), inositol bisphosphate (IP2), and inositol trisphosphate (IP3) in a single run. In GH3 cells labelled with [3H]inositol, treated with Li+ and thyrotropin-releasing hormone (TRH), radiolabelled components identified as inositol 1-phosphate (I1P), inositol 2-phosphate (I2P), inositol 4-phosphate (I4P), inositol 1,4-bisphosphate [I(1,4)P2], inositol 1,3,4-trisphosphate [I(1,3,4)P3] and inositol 1,4,5-trisphosphate [I(1,4,5)P3] are present, as are multiple unidentified IP2 peaks. After TRH stimulation, both I1P and I4P increase, the increase in I4P preceding that of I1P; I(1,4)P2 and an unknown IP2 increase; and both I(1,3,4)P3 and I(1,4,5)P3 increase, the increase in I(1,4,5)P3 being rapid and transient, whereas the increase in I(1,3,4)P3 is slower and more sustained. The most rapidly appearing inositol phosphates produced after TRH stimulation are I(1,4)P2 and I(1,4,5)P3.

摘要

已开发出一种高效液相色谱（h.p.l.c.）分离方法，可在一次运行中分离肌醇单磷酸（IP）、肌醇二磷酸（IP2）和肌醇三磷酸（IP3）的异构体。在用[3H]肌醇标记的GH3细胞中，用Li+和促甲状腺激素释放激素（TRH）处理后，鉴定出的放射性标记成分有肌醇1-磷酸（I1P）、肌醇2-磷酸（I2P）、肌醇4-磷酸（I4P）、肌醇1,4-二磷酸[I(1,4)P2]、肌醇1,3,4-三磷酸[I(1,3,4)P3]和肌醇1,4,5-三磷酸[I(1,4,5)P3]，还有多个未鉴定的IP2峰。TRH刺激后，I1P和I4P均增加，I4P的增加先于I1P；I(1,4)P2和一种未知的IP2增加；I(1,3,4)P3和I(1,4,5)P3均增加，I(1,4,5)P3的增加迅速且短暂，而I(1,3,4)P3的增加较慢且更持久。TRH刺激后产生的出现最快的肌醇磷酸是I(1,4)P2和I(1,4,5)P3。

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