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2
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本文引用的文献

1
Purification and properties of a human platelet inositol 1,4,5-trisphosphate 3-kinase.人血小板肌醇-1,4,5-三磷酸3-激酶的纯化及性质
Arch Biochem Biophys. 1993 Jun;303(2):412-20. doi: 10.1006/abbi.1993.1303.
2
Isolation and sequence of a full length cDNA encoding a novel rat inositol 1,4,5-trisphosphate 3-kinase.编码一种新型大鼠肌醇1,4,5-三磷酸3-激酶的全长cDNA的分离与测序
Biochim Biophys Acta. 1994 Jan 13;1220(2):219-22. doi: 10.1016/0167-4889(94)90139-2.
3
Purification and biochemical properties of a high-molecular-mass inositol 1,4,5-trisphosphate 3-kinase isoenzyme in human platelets.人血小板中一种高分子量肌醇1,4,5-三磷酸3-激酶同工酶的纯化及生化特性
Biochem J. 1994 Mar 15;298 Pt 3(Pt 3):669-73. doi: 10.1042/bj2980669.
4
Golgi coatomer binds, and forms K(+)-selective channels gated by, inositol polyphosphates.高尔基体衣被蛋白结合肌醇多磷酸并形成由其门控的钾离子选择性通道。
J Biol Chem. 1994 Jul 8;269(27):17826-32.
5
Purification and characterization of recombinant human p50csk protein-tyrosine kinase from an Escherichia coli expression system overproducing the bacterial chaperones GroES and GroEL.从过量表达细菌伴侣蛋白GroES和GroEL的大肠杆菌表达系统中纯化和鉴定重组人p50csk蛋白酪氨酸激酶。
Proc Natl Acad Sci U S A. 1995 Feb 14;92(4):1048-52. doi: 10.1073/pnas.92.4.1048.
6
Inhibition of clathrin assembly by high affinity binding of specific inositol polyphosphates to the synapse-specific clathrin assembly protein AP-3.特定肌醇多磷酸与突触特异性网格蛋白组装蛋白AP-3的高亲和力结合对网格蛋白组装的抑制作用。
J Biol Chem. 1995 Jan 27;270(4):1564-8.
7
Purification and characterization of an Ins(1,3,4,5)P4 binding protein from pig platelets: possible identification of a novel non-neuronal Ins(1,3,4,5)P4 receptor.猪血小板中 Ins(1,3,4,5)P4 结合蛋白的纯化与特性分析：一种新型非神经元 Ins(1,3,4,5)P4 受体的可能鉴定
Biochem J. 1995 Jan 1;305 ( Pt 1)(Pt 1):139-43. doi: 10.1042/bj3050139.
8
Identification of a specific Ins(1,3,4,5)P4-binding protein as a member of the GAP1 family.鉴定一种特定的1,3,4,5-肌醇四磷酸结合蛋白为GAP1家族的成员。
Nature. 1995 Aug 10;376(6540):527-30. doi: 10.1038/376527a0.
9
Regulation of inositol 1,4,5-trisphosphate kinase activity after stimulation of human T cell antigen receptor.人T细胞抗原受体刺激后肌醇1,4,5-三磷酸激酶活性的调节
J Clin Invest. 1987 May;79(5):1538-41. doi: 10.1172/JCI112986.
10
Separation of multiple isomers of inositol phosphates formed in GH3 cells.GH3细胞中形成的肌醇磷酸多种异构体的分离。
Biochem J. 1987 Mar 1;242(2):361-6. doi: 10.1042/bj2420361.

重组大鼠肌醇-1,4,5-三磷酸3-激酶B的表达表明其N端具有调节作用。

Expression of recombinant rat myo-inositol 1,4,5-trisphosphate 3-kinase B suggests a regulatory role for its N-terminus.

作者信息

Thomas S, Soriano S, d'Santos C, Banting G

机构信息

Department of Biochemistry, University of Bristol, U.K.

出版信息

Biochem J. 1996 Nov 1;319 ( Pt 3)(Pt 3):713-6. doi: 10.1042/bj3190713.

DOI:10.1042/bj3190713
PMID:8920971
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1217847/
Abstract

We have expressed rat myo-inositol 1,4,5-trisphosphate (IP3) 3-kinase B as both a full-length, recombinant, non-fusion protein and a full-length, recombinant, fusion protein with maltose-binding protein (MBP) in Escherichia coli. The fusion protein with MBP is soluble, binds calmodulin and is enzymically active whereas the non-fusion protein is insoluble and does not bind calmodulin unless co-expressed with bacterial chaperone proteins (either GroES and GroEL, or DnaK, DnaJ and GrpE). However, soluble, calmodulin-binding non-fusion IP3 3-kinase B is enzymically inactive. The catalytic domain of the enzyme has previously been shown to reside near the C-terminus; the results we present suggest an auto-regulatory role for the N-terminus.

摘要

我们已在大肠杆菌中表达了大鼠肌醇1,4,5-三磷酸（IP3）3-激酶B，其形式包括全长重组非融合蛋白以及与麦芽糖结合蛋白（MBP）的全长重组融合蛋白。与MBP的融合蛋白是可溶的，能结合钙调蛋白且具有酶活性，而该非融合蛋白是不溶的，除非与细菌伴侣蛋白（GroES和GroEL，或DnaK、DnaJ和GrpE）共表达，否则不结合钙调蛋白。然而，可溶的、结合钙调蛋白的非融合IP3 3-激酶B没有酶活性。此前已表明该酶的催化结构域位于C端附近；我们给出的结果表明N端具有自动调节作用。