College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China.
Food Funct. 2019 Jun 19;10(6):3282-3290. doi: 10.1039/c8fo02396d.
In the present study, the hypoglycemic effects of wheat bran alkyresorcinols (ARs) were investigated in type 2 diabetes mellitus (T2DM) mice induced by a high-fat/high-sucrose diet (HFSD) combined with low dose streptozotocin (STZ). After the consumption of 5 mg kg-1 d-1 acarbose (positive control) and different doses of wheat bran ARs (50, 200 and 500 mg kg-1 d-1) for 4 weeks, the fasting blood glucose (FBG) levels in T2DM mice were found to be reduced significantly (p < 0.05), and the effects of 200 and 500 mg kg-1 d-1 administration were better than that of 50 mg kg-1 d-1. The results of the oral glucose tolerance test (OGTT) also showed that both acarbose and AR administration significantly increased the glucose tolerance of the T2DM mice. Then, the fasting serum insulin levels (FINS) were significantly reduced by AR treatment, and the effect of 500 mg kg-1 d-1 AR administration was better than that of 5 mg kg-1 d-1 acarbose. The profile of plasma lipids was analyzed simultaneously, and the results showed that the contents of total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol (LDL-C) were significantly decreased, while the content of high-density lipoprotein cholesterol (HDL-C) was increased significantly after 200 and 500 mg kg-1 d-1 AR treatment (p < 0.05). Furthermore, 200 and 500 mg kg-1 d-1 ARs significantly increased the content of hepatic glycogen and the activity of glucokinase (p < 0.01) in T2DM mice. The relative mRNA levels of glucose transporter 2 (GLUT2) in the liver tissue were increased markedly in 200 and 500 mg kg-1 d-1 AR treatment groups (p < 0.01), and the relative mRNA levels of glucose transporter 4 (GLUT4) in the epididymal adipose tissue were increased significantly in all AR treatment groups, especially significantly higher than acarbose (p < 0.01). Histological analyses revealed that treatment with ARs exerted a protective role on pancreatic β-cells. The results indicated that ARs could be an effective hypoglycemic active ingredient in whole grain diets.
在本研究中，研究了小麦麸烷基间苯二酚（ARs）在高脂肪/高蔗糖饮食（HFSD）联合低剂量链脲佐菌素（STZ）诱导的 2 型糖尿病（T2DM）小鼠中的降血糖作用。连续 4 周给予 5mg/kg-1d-1阿卡波糖（阳性对照）和不同剂量的小麦麸 ARs（50、200 和 500mg/kg-1d-1）后，T2DM 小鼠的空腹血糖（FBG）水平明显降低（p<0.05），且 200 和 500mg/kg-1d-1 给药的效果优于 50mg/kg-1d-1。口服葡萄糖耐量试验（OGTT）的结果也表明，阿卡波糖和 AR 给药均显著提高了 T2DM 小鼠的葡萄糖耐量。然后，AR 处理显著降低了空腹血清胰岛素水平（FINS），且 500mg/kg-1d-1 AR 给药的效果优于 5mg/kg-1d-1 阿卡波糖。同时分析了血浆脂质谱，结果表明，200 和 500mg/kg-1d-1 AR 处理后，总胆固醇（TC）、甘油三酯（TG）和低密度脂蛋白胆固醇（LDL-C）的含量显著降低，而高密度脂蛋白胆固醇（HDL-C）的含量显著增加（p<0.05）。此外，200 和 500mg/kg-1d-1 ARs 显著增加了 T2DM 小鼠肝糖原的含量和葡萄糖激酶（GK）的活性（p<0.01）。200 和 500mg/kg-1d-1 AR 处理组肝组织中葡萄糖转运蛋白 2（GLUT2）的相对 mRNA 水平显著升高（p<0.01），所有 AR 处理组附睾脂肪组织中葡萄糖转运蛋白 4（GLUT4）的相对 mRNA 水平显著升高，明显高于阿卡波糖（p<0.01）。组织学分析表明，ARs 对胰腺β细胞具有保护作用。结果表明，ARs 可能是全谷物饮食中一种有效的降血糖活性成分。
