State Key Laboratory of Urban and Regional Ecology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Beijing 100085, China.
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen 361021, China; University of the Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
Sci Total Environ. 2019 Apr 1;659:715-723. doi: 10.1016/j.scitotenv.2018.12.445. Epub 2018 Dec 29.
Liming is a common agronomic practice used for alleviating soil acidification to improve plant growth. However, it is still unclear how liming can affect the gut microbiota composition of soil fauna, and subsequently the nutrient cycling and litter decomposition mediated by soil fauna. In the present study the effect of liming on the gut microbiota of two types of soil fauna, Folsomia candida, and Enchytraeus crypticus was investigated by using 16S rRNA gene high-throughput sequencing. The results revealed that there are differences between the gut microbial communities of the two types of soil fauna as well as between the gut microbiome of the soil fauna and the surrounding soil. Enterobacteriaceae and Bacillaceae were the predominant families in the gut microbiota of E. crypticus, while Rickettsiaceae and Moraxellaceae were the predominant families in the gut microbiota of F. candida. Liming affected the gut microbiota of E. crypticus at both the taxonomical and core microbiota level. The gut microbiota of F. candida was not affected by liming. Structural equation models suggest that 97% of the variation in the E. crypticus gut microbiota could be explained by liming-induced changes in soil properties and the soil microbial community. The indirect effects of liming, caused by a shift in the soil microbial community, contributed more in reshaping the gut microbiota of E. crypticus than the direct effects of the changed soil properties did. These findings suggest that the effects of liming on the gut microbiota composition in soil fauna are species-specific and are likely dependent on the response of the host to changes in soil properties and the soil microbial community.
石灰处理是一种常用的农业措施，用于缓解土壤酸化，以改善植物生长。然而，石灰处理如何影响土壤动物肠道微生物群落，以及随后受土壤动物介导的养分循环和凋落物分解，目前仍不清楚。在本研究中，通过使用 16S rRNA 基因高通量测序，研究了石灰处理对两种土壤动物——弗氏真涡虫（Folsomia candida）和长尾真涡蚓（Enchytraeus crypticus）肠道微生物群落的影响。结果表明，两种土壤动物的肠道微生物群落之间以及土壤动物肠道微生物群落与周围土壤之间存在差异。肠杆菌科（Enterobacteriaceae）和芽孢杆菌科（Bacillaceae）是长尾真涡蚓肠道微生物群落中的主要科，而赖氏菌科（Rickettsiaceae）和莫拉氏菌科（Moraxellaceae）是弗氏真涡虫肠道微生物群落中的主要科。石灰处理影响长尾真涡蚓的肠道微生物群落，在分类学和核心微生物群水平上均有影响。石灰处理对弗氏真涡虫的肠道微生物群落没有影响。结构方程模型表明，石灰处理引起的土壤性质和土壤微生物群落变化可以解释长尾真涡蚓肠道微生物群落 97%的变异。土壤微生物群落变化引起的间接效应对重塑长尾真涡蚓肠道微生物群落的贡献大于土壤性质变化引起的直接效应。这些发现表明，石灰处理对土壤动物肠道微生物群落组成的影响是物种特异性的，可能取决于宿主对土壤性质和土壤微生物群落变化的反应。
