Department of Microbiology and Immunology, School of Medicine, Stanford University, Palo Alto, 94305, United States; Department of Animal Science, College of Agriculture and Life Science, Cornell University, Ithaca, 14853, United States.
Department of Animal Science, College of Agriculture and Life Science, Cornell University, Ithaca, 14853, United States.
Environ Pollut. 2018 Aug;239:408-415. doi: 10.1016/j.envpol.2018.04.017. Epub 2018 Apr 19.
Microplastics are emerging pollutants that have recently aroused considerable concern but most toxicological studies have focused on marine biota, with little investigation of the influence of microplastics on terrestrial ecosystems. Here, we fed the soil oligochaete Enchytraeus crypticus with oatmeal containing 0, 0.025, 0.5, and 10% (dry weight basis) nano-polystyrene (0.05-0.1 μm particle size) to elucidate the impact of microplastics on the growth and gut microbiome of Enchytraeus crypticus. We observed a significant reduction of weight in the animals fed 10% polystyrene and an increase in the reproduction of those fed 0.025%. More importantly, using 16S rRNA amplification and high-throughput sequencing we found a significant shift in the microbiome of those fed 10% microplastics with significant decreases in the relative abundance of the families Rhizobiaceae, Xanthobacteraceae and Isosphaeraceae. These families contain key microbes that contribute to nitrogen cycling and organic matter decomposition.
微塑料是新兴的污染物，最近引起了相当大的关注，但大多数毒理学研究都集中在海洋生物群上，而对微塑料对陆地生态系统的影响的研究较少。在这里，我们用含有 0、0.025、0.5 和 10%（干重基础）纳米聚苯乙烯（粒径 0.05-0.1μm）的燕麦片喂养土壤寡毛类环节动物秀丽隐杆线虫，以阐明微塑料对秀丽隐杆线虫生长和肠道微生物组的影响。我们观察到，喂食 10%聚苯乙烯的动物体重明显减轻，而喂食 0.025%聚苯乙烯的动物繁殖增加。更重要的是，使用 16S rRNA 扩增和高通量测序，我们发现喂食 10%微塑料的动物的微生物组发生了显著变化，根瘤菌科、黄杆菌科和等孢菌科的相对丰度显著降低。这些科包含有助于氮循环和有机质分解的关键微生物。
