Institute for Organic Chemistry and Centre for Biomolecular Drug Research , Leibniz University Hannover , Schneiderberg 38 , Hannover 30167 , Germany.
Org Lett. 2019 Jun 7;21(11):4163-4167. doi: 10.1021/acs.orglett.9b01344. Epub 2019 May 17.
The biosynthetic gene cluster encoding the phytotoxin pyrichalasin H 5 was discovered in Magnaporthe grisea NI980, and the late-stage biosynthetic pathway of 5 was fully elucidated using targeted gene inactivations resulting in the isolation of 13 novel cytochalasans. This study reveals that the nonproteinogenic amino acid O-methyltyrosine is the true precursor of 5, and other cryptic cytochalasans and mutasynthesis experiments produce novel halogenated pyrichalasin H analogues.
在稻瘟病菌 NI980 中发现了编码植物毒素吡里恰尔森 H5 的生物合成基因簇，并通过靶向基因失活充分阐明了 5 的后期生物合成途径，从而分离出 13 种新型细胞松弛素。本研究表明，非蛋白氨基酸 O-甲基酪氨酸是 5 的真正前体，其他隐色细胞松弛素和突变合成实验产生了新型卤代吡里恰尔森 H 类似物。
