Hantke Verena, Wang Chongqing, Skellam Elizabeth J, Cox Russell J
Institute for Organic Chemistry and BMWZ, Leibniz Universität Hannover Schneiderberg 38 30167 Hannover Germany
RSC Adv. 2019 Nov 4;9(61):35797-35802. doi: 10.1039/c9ra07028a. eCollection 2019 Oct 31.
Ectopic expression of which encodes a putative pathway specific transcription factor from the biosynthetic gene cluster of the rice pathogen Guy11 did not lead to the production of -related compounds. However the known compound hinnulin A was formed. A putative partial gene cluster potentially involved in the biosynthesis of hinnulin A and DHN melanin was validated by RT-PCR and a possible biosynthetic pathway is proposed. Ectopic expression of which encodes a pathway specific transcription factor from the pyrichalasin H biosynthetic gene cluster in NI980 led to the apparent up-regulation of the cluster and a 3-fold increase in pyrichalasin production under standard fermentation conditions, but did not lead to the formation of new compounds.
编码来自水稻病原菌稻瘟病菌Guy11生物合成基因簇中假定的途径特异性转录因子的异位表达，并未导致与该途径相关化合物的产生。然而，已知化合物hinnulin A形成了。通过RT-PCR验证了一个可能参与hinnulin A和DHN黑色素生物合成的假定部分基因簇，并提出了一条可能的生物合成途径。编码来自NI980中吡咯菌素H生物合成基因簇的途径特异性转录因子的异位表达，导致该基因簇明显上调，并且在标准发酵条件下吡咯菌素产量增加了3倍，但未导致新化合物的形成。
