Function of pathway specific regulators in the and pyrichalasin H biosynthetic gene clusters.

Ectopic expression of which encodes a putative pathway specific transcription factor from the biosynthetic gene cluster of the rice pathogen Guy11 did not lead to the production of -related compounds. However the known compound hinnulin A was formed. A putative partial gene cluster potentially involved in the biosynthesis of hinnulin A and DHN melanin was validated by RT-PCR and a possible biosynthetic pathway is proposed. Ectopic expression of which encodes a pathway specific transcription factor from the pyrichalasin H biosynthetic gene cluster in NI980 led to the apparent up-regulation of the cluster and a 3-fold increase in pyrichalasin production under standard fermentation conditions, but did not lead to the formation of new compounds.