Heo Jun, Cho Hayoung, Kim Mi Ae, Hamada Moriyuki, Tamura Tomohiko, Saitou Satomi, Kim Soo-Jin, Kwon Soon-Wo
1Agricultural Microbiology Division, National Institute of Agricultural Science, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea.
2Industrial Insect Division, National Institute of Agricultural Science, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea.
Int J Syst Evol Microbiol. 2019 Jul;69(7):2101-2107. doi: 10.1099/ijsem.0.003444. Epub 2019 May 17.
A Gram-stain-positive, strictly aerobic, rod-shaped, non-spore-forming, non-motile bacterium, designated strain 2DFWR-13, was isolated from gut of the larva of Protaetia brevitarsis seulensis, in the Republic of Korea. Strain 2DFWR-13 showed high sequence similarities to Lysinimonas kribbensis MSL-13 (97.7 %), Homoserinibacter gongjuensis 5GH26-15 (97.2 %), Microbacterium deminutum KV-483 (97.1 %) and Herbiconiux ginsengi CGMCC 4.3491 (97.1 %). The predominant fatty acids (>10 % of the total fatty acids) were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0. The major menaquinones were MK-13 and MK-12. The peptidoglycan type was type B2 with the diagnostic amino acid d-DAB. The N-acyl type of the murein was glycolyl. The polar lipids consisted of diphosphatidylglycerol, an unidentified glycolipid and an unidentified lipid. The DNA G+C content was 71.5 mol%. Based on its phylogenetic distinctiveness and distinguishing phenotypic characteristics, we conclude that strain 2DFWR-13 represents a novel genus and species of the family Microbacteriaceae, for which the name Protaetiibacter intestinalis gen. nov., sp. nov. is proposed. The type strain of Protaetiibacter intestinalis is 2DFWR-13 (=KACC 19321=NBRC 113050). In addition, an emended description of the genus LysinimonasJang et al. 2013 and the reclassification of Lysinimonas kribbensisJang et al. 2013 as Pseudolysinimonas kribbensis gen. nov., comb. nov. are proposed.
从韩国短角扁犀金龟幼虫肠道中分离出一株革兰氏染色阳性、严格需氧、杆状、不产芽孢、无运动性的细菌，命名为菌株2DFWR-13。菌株2DFWR-13与克氏赖氨酸单胞菌MSL-13（97.7%）、公州高丝氨酸单胞菌5GH26-15（97.2%）、微小微杆菌KV-483（97.1%）和人参草螺菌CGMCC 4.3491（97.1%）显示出高度的序列相似性。主要脂肪酸（占总脂肪酸的>10%）为anteiso-C15 : 0、iso-C16 : 0和anteiso-C17 : 0。主要甲基萘醌为MK-13和MK-12。肽聚糖类型为B2型，诊断性氨基酸为d-DAB。胞壁质的N-酰基类型为糖基。极性脂质由双磷脂酰甘油、一种未鉴定的糖脂和一种未鉴定的脂质组成。DNA G+C含量为71.5 mol%。基于其系统发育独特性和显著的表型特征，我们得出结论，菌株2DFWR-13代表微杆菌科的一个新属和新种，为此提出新属名肠道扁犀金龟菌属（Protaetiibacter）、新种名肠道扁犀金龟菌（Protaetiibacter intestinalis）。肠道扁犀金龟菌的模式菌株为2DFWR-13（=KACC 19321=NBRC 113050）。此外，还提出了对赖氨酸单胞菌属（Lysinimonas）Jang等人（2013年）的修订描述，以及将克氏赖氨酸单胞菌Jang等人（2013年）重新分类为新组合的假赖氨酸单胞菌（Pseudolysinimonas kribbensis）。
