Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 565-851, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Feb;70(2):989-994. doi: 10.1099/ijsem.0.003860.
A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea. Growth was observed at 15-50 °C (optimum, 28-37 °C), pH 6.0-8.0 (pH 7.0) and only without NaCl. 16S rRNA gene sequence comparisons indicated that strain FW100M-2 had the highest similarity to type strains of S3-4A (96.8 %) and DSM 1355 (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa. The phylogenetic tree showed that strain FW100M-2 fell into the genus , and formed a cluster with S3-4A independent from other species. Antesio-C, iso-C and anteiso-C were detected as the major fatty acids. The only isoprenoid quinone was MK-7. Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid were present. The -diaminopimelic acid was present in the cell-wall peptidoglycan. The genomic DNA G+C content was 51.5 mol%. Hence, strain FW100M-2 represents a novel species of the genus , for which the name sp. nov. is proposed, with FW100M-2 (=KACC 19327=NBRC 113071) as the type strain.
一株革兰氏阳性、杆状、严格需氧、芽孢内生、周生鞭毛的细菌，从韩国全罗南道务安郡国立农业科学院幼虫的肠道样本中分离得到。在 15-50°C（最佳温度 28-37°C）、pH 6.0-8.0（pH 7.0）和不含 NaCl 的条件下可以观察到生长。16S rRNA 基因序列比较表明，FW100M-2 株与 S3-4A（96.8%）和 DSM 1355（96.3%）的模式菌株具有最高的相似性，与所有其他分类群的序列相似性值均小于 96.0%。系统发育树显示，FW100M-2 株属于 属，与 S3-4A 一起形成了一个与其他 种独立的聚类。anteiso-C、iso-C 和 anteiso-C 被检测为主要脂肪酸。唯一的异戊烯醌是 MK-7。极性脂包括双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的氨基磷脂、两种未鉴定的磷脂和一种未鉴定的脂类。细胞壁肽聚糖中存在 -二氨基庚二酸。基因组 DNA G+C 含量为 51.5mol%。因此，FW100M-2 株代表了 属的一个新种，提议使用 FW100M-2（=KACC 19327=NBRC 113071）作为模式株，命名为 sp. nov.。
