Chakrabarti S, Sima A A, Nakajima T, Yagihashi S, Greene D A
Diabetologia. 1987 Apr;30(4):244-51. doi: 10.1007/BF00270423.
Aldose reductase was purified from testis of non-diabetic BB rats using DEAE cellulose, hydroxylapatite and sephadex G-100 column chromatography. The molecular weight of the isolated enzyme was found to be 36,500 +/- 1000. Antibody against the isolated enzyme was raised in rabbits. It was purified by affinity chromatography, characterised by double immunodiffusion and Western blot analysis and used to localize the enzyme in retina and in peripheral nerve of the BB rat. In the retina, aldose reductase immunoreactivity was seen in the ganglion cells, Müller cell processes, retinal pigment epithelium and in the pericytes and endothelial cells of retinal capillaries. In peripheral nerve, aldose reductase immunoreactivity was found in the paranodal cytoplasm of Schwann cells and in pericytes and endothelial cells of endoneurial capillaries.
使用DEAE纤维素、羟基磷灰石和葡聚糖G - 100柱色谱法从非糖尿病BB大鼠的睾丸中纯化醛糖还原酶。发现分离出的酶的分子量为36,500±1000。在兔体内制备针对分离出的酶的抗体。通过亲和色谱法对其进行纯化，通过双向免疫扩散和蛋白质印迹分析对其进行表征，并用于在BB大鼠的视网膜和外周神经中定位该酶。在视网膜中，在神经节细胞、穆勒细胞突起、视网膜色素上皮以及视网膜毛细血管的周细胞和内皮细胞中可见醛糖还原酶免疫反应性。在外周神经中，在雪旺细胞的结旁细胞质以及神经内膜毛细血管的周细胞和内皮细胞中发现醛糖还原酶免疫反应性。
