Fusaoctaxin A, an Example of a Two-Step Mechanism for Non-Ribosomal Peptide Assembly and Maturation in Fungi.

Fungal non-ribosomal peptide synthetase ( clusters are spread across the chromosomes, where several modifying enzyme-encoding genes typically flank one . However, a recent study showed that the octapeptide fusaoctaxin A is tandemly synthesized by two NRPSs in . Here, we illuminate parts of the biosynthetic route of fusaoctaxin A, which is cleaved into the tripeptide fusatrixin A and the pentapeptide fusapentaxin A during transport by a cluster-specific ABC transporter with peptidase activity. Further, we deleted the histone H3K27 methyltransferase , which induced the production of fusaoctaxin A.