Faculty of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, 40032 Katowice, Poland.
Faculty of Engineering and Science, Department of Chemistry and Biosciences, University of Aalborg, 9220 Aalborg, Denmark.
Int J Mol Sci. 2021 Jul 12;22(14):7441. doi: 10.3390/ijms22147441.
Crop infections by fungi lead to severe losses in food production and pose risks for human health. The increasing resistance of pathogens to fungicides has led to the higher usage of these chemicals, which burdens the environment and highlights the need to find novel natural biocontrol agents. Members of the genus are known to produce a plethora of bioactive compounds. Recently, researchers have turned to extreme and previously unexplored niches in the search for new strains with antimicrobial activities. One such niche are underground coal mine environments. We isolated the new sp. MW-W600-10 strain from coal mine water samples collected at 665 m below ground level. We examined the antifungal activity of the strain against plant pathogens DSM62188 and roseF7. Furthermore, we analyzed the strain's biosynthetic potential with the antiSMASH tool. The strain showed inhibitory activity against both fungi strains. Genome mining revealed that it has 39 BGCs, among which 13 did not show similarity to those in databases. Additionally, we examined the activity of the sp. S-2 strain isolated from black soot against DSM62188. These results show that coal-related strains could be a source of novel bioactive compounds. Future studies will elucidate their full biotechnological potential.
真菌对农作物的感染导致了粮食生产的严重损失，并对人类健康构成了威胁。病原体对杀菌剂的抗性不断增加，导致这些化学物质的使用量增加，给环境带来了负担，也凸显了寻找新型天然生物防治剂的必要性。已知属的成员能够产生大量的生物活性化合物。最近，研究人员开始在极端和以前未探索过的小生境中寻找具有抗菌活性的新菌株。其中一个小生境是地下煤矿环境。我们从地下 665 米处采集的煤矿水样中分离出了新型 sp. MW-W600-10 菌株。我们研究了该菌株对植物病原菌 DSM62188 和 roseF7 的抗真菌活性。此外，我们还使用 antiSMASH 工具分析了该菌株的生物合成潜力。该菌株对两种真菌均显示出抑制活性。基因组挖掘显示，它有 39 个 BGCs，其中 13 个与数据库中的没有相似性。此外，我们还研究了从黑煤灰中分离出的 sp. S-2 菌株对 DSM62188 的活性。这些结果表明，与煤炭相关的菌株可能是新型生物活性化合物的来源。未来的研究将阐明它们的全部生物技术潜力。
