Jang Eunhye, Lee Ja-Youn, Lee Eun-Young, Seok Hyun
Department of Orthodontics, College of Dentistry, Gangneung-Wonju National University, Gangneung 25457, Korea.
Department of Prosthodontics, Chungbuk National University Hospital, Cheongju 28644, Korea.
Materials (Basel). 2019 May 16;12(10):1613. doi: 10.3390/ma12101613.
The aim of this study was to evaluate the bone regeneration effect of recombinant human bone morphogenetic protein-2 (rhBMP-2) on a subperiosteal bone graft in a rat model. A subperiosteal space was made on the rat calvarium, and anorganic bovine bone (ABB), ABB/low bone morphogenetic protein (BMP) (5 µg), and ABB/high BMP (50 µg) were grafted as subperiosteal bone grafts. The new bone formation parameters of bone volume (BV), bone mineral density (BMD), trabecular thickness (TbTh), and trabecular spacing (TbSp) were evaluated by microcomputed tomography (µ-CT), and a histomorphometric analysis was performed to evaluate the new bone formation area. The expression of osteogenic markers, such as bone sialoprotein (BSP) and osteocalcin, were evaluated by immunohistochemistry (IHC). The ABB/high BMP group showed significantly higher BV than the ABB/low BMP ( = 0.004) and control groups ( = 0.000) and higher TbTh than the control group ( = 0.000). The ABB/low BMP group showed significantly higher BV, BMD, and TbTh than the control group ( = 0.002, 0.042, and 0.000, respectively). The histomorphometry showed significantly higher bone formation in the ABB/low and high BMP groups than in the control group ( = 0.000). IHC showed a high expression of BSP and osteocalcin in the ABB/low and high BMP groups. Subperiosteal bone grafts with ABB and rhBMP-2 have not been studied. In our study, we confirmed that rhBMP-2 contributes to new bone formation in a subperiosteal bone graft with ABB.
本研究的目的是在大鼠模型中评估重组人骨形态发生蛋白-2（rhBMP-2）对骨膜下骨移植的骨再生作用。在大鼠颅骨上制作骨膜下间隙，并将无机牛骨（ABB）、ABB/低骨形态发生蛋白（BMP）（5μg）和ABB/高BMP（50μg）作为骨膜下骨移植材料进行移植。通过微型计算机断层扫描（µ-CT）评估骨体积（BV）、骨矿物质密度（BMD）、小梁厚度（TbTh）和小梁间距（TbSp）等新骨形成参数，并进行组织形态计量学分析以评估新骨形成面积。通过免疫组织化学（IHC）评估骨涎蛋白（BSP）和骨钙素等成骨标志物的表达。ABB/高BMP组的BV显著高于ABB/低BMP组（P = 0.004）和对照组（P = 0.000），TbTh高于对照组（P = 0.000）。ABB/低BMP组的BV、BMD和TbTh显著高于对照组（分别为P = 0.002、0.042和0.000）。组织形态计量学显示，ABB/低BMP组和高BMP组的骨形成显著高于对照组（P = 0.000）。免疫组织化学显示，ABB/低BMP组和高BMP组中BSP和骨钙素表达较高。尚未对含有ABB和rhBMP-2的骨膜下骨移植进行研究。在我们的研究中，我们证实rhBMP-2有助于含ABB的骨膜下骨移植中的新骨形成。
