Department of Periodontology, School of Dentistry, Universidade Federal de Uberlândia - UFU, Uberlândia, MG, Brazil.
Department of Diagnosis and Surgery, School of Dentistry, Universidade Estadual Paulista - UNESP, Araraquara, SP, Brazil.
Braz Dent J. 2022 Jul-Aug;33(4):40-46. doi: 10.1590/0103-6440202204823.
The study aimed to evaluate the accuracy of Micro-CT in linear and volumetric measurements in native (NB) and grafted bone (GB) areas. A total of 111 biopsies of maxillary sinuses grafted with deproteinized bovine bone (DBB) in humans were evaluated. The linear measurements were performed to measure the length of the NB and GB. Furthermore, the amount of mineralized tissues at the NB and GB was performed. In the histomorphometry analysis the percentage of mineralized tissues at the NB and GB was obtained in two histological sections while the mineralized tissues were measure in the micro-CT varying the thresholds of the grayscale varying from 90-250 to 90-150 with 10 levels of variation between each one was applied. Then these data were correlated in order to check the higher r level between the histomorphometry and micro-CT thresholds intervals. The linear length of the NB was 2.44±0.91mm and 2.48±1.50mm, respectively, for micro-CT and histomorphometry (r =0.57), while the linear length of the GB was 3.63±1.66mm and 3.13±1.45mm, respectively, for micro-CT and histomorphometry (r =0.74) Histomorphometry showed 45.91±11.69% of bone in NB, and 49.57±5.59% of bone and biomaterial in the GB. The total volume of mineralized tissues that were closest to the histometric analysis were 43.75±15.39% in the NB (Threshold:90-240; r = 0.50) and 51.68±8.42% in the GB (Threshold:90-180; r =-0.028). The micro-CT analysis showed good accuracy in the linear analysis in both portions of the biopsies but for volumetric analysis just in NB.
本研究旨在评估 Micro-CT 在测量天然（NB）和移植骨（GB）区域的线性和体积方面的准确性。共评估了 111 例经脱蛋白牛骨（DBB）移植的上颌窦活检。进行线性测量以测量 NB 和 GB 的长度。此外，还对 NB 和 GB 的矿化组织量进行了测量。在组织形态计量学分析中，在两个组织学切片中获得了 NB 和 GB 矿化组织的百分比，而在 Micro-CT 中，通过在 90-250 到 90-150 的灰度阈值之间变化 10 个水平来测量矿化组织。然后将这些数据进行相关分析，以检查组织形态计量学和 Micro-CT 阈值间隔之间的更高 r 水平。NB 的线性长度分别为 Micro-CT 和组织形态计量学的 2.44±0.91mm 和 2.48±1.50mm（r =0.57），而 GB 的线性长度分别为 Micro-CT 和组织形态计量学的 3.63±1.66mm 和 3.13±1.45mm（r =0.74）。组织形态计量学显示 NB 中骨的含量为 45.91±11.69%，GB 中骨和生物材料的含量为 49.57±5.59%。与组织学分析最接近的矿化组织总体积分别为 NB 中的 43.75±15.39%（阈值：90-240；r =0.50）和 GB 中的 51.68±8.42%（阈值：90-180；r =-0.028）。Micro-CT 分析显示在活检的两个部分的线性分析中具有良好的准确性，但在体积分析中仅在 NB 中有效。
