Varma S D, Bauer S A, Richards R D
Invest Ophthalmol Vis Sci. 1987 Jul;28(7):1164-9.
The metabolism of glucose through the hexose monophosphate (HMP) shunt has been studied in rat lens in vitro, in the absence and presence of ascorbic and dehydroascorbic acids. Both forms of the vitamin stimulated the utilization of glucose through the HMP shunt, the stimulatory effect of dehydroascorbate being substantially greater than that of ascorbate. The stimulatory effect of ascorbate, as well as of dehydroascorbate, was antagonized by sodium iodide, and N,N-bis (dichloroethyl)-N-nitrosourea, compounds known to inhibit glutathione reductase. N-ethylmaleimide also antagonized the stimulation. These findings, therefore, suggest that the DHA/AA redox couple acts in concert with the GSSG/GSH couple in stimulating the tissue shunt activity.
在体外培养的大鼠晶状体中，研究了在有无抗坏血酸和脱氢抗坏血酸的情况下，葡萄糖通过己糖磷酸（HMP）支路的代谢情况。这两种形式的维生素都能刺激葡萄糖通过HMP支路的利用，脱氢抗坏血酸盐的刺激作用明显大于抗坏血酸盐。抗坏血酸盐和脱氢抗坏血酸盐的刺激作用都被碘化钠和N，N-双（二氯乙基）-N-亚硝基脲所拮抗，这两种化合物已知能抑制谷胱甘肽还原酶。N-乙基马来酰亚胺也能拮抗这种刺激作用。因此，这些发现表明，DHA/AA氧化还原对与GSSG/GSH对协同作用，刺激组织支路活性。
