黄芩苷对溃疡性结肠炎大鼠 IKK/IKB/NF-κB 信号通路及凋亡相关蛋白的调节作用。
Regulating effect of baicalin on IKK/IKB/NF-kB signaling pathway and apoptosis-related proteins in rats with ulcerative colitis.
机构信息
Department of Pediatrics, Shuguang Hospital Affiliated to Shanghai Traditional Chinese Medical University, Shanghai 201203, China.
Department of Pediatrics, Shuguang Hospital Affiliated to Shanghai Traditional Chinese Medical University, Shanghai 201203, China.
出版信息
Int Immunopharmacol. 2019 Aug;73:193-200. doi: 10.1016/j.intimp.2019.04.052. Epub 2019 May 16.
OBJECTIVE
This study was aimed to explore effect of baicalin on IKK/IKB/NF-kB signaling pathway and apoptosis-related proteins in rats with ulcerative colitis (UC).
METHODS
Histopathological observation and scores of colon tissue were performed in the UC rat model. IKK/IKB/NF-kB signaling pathway and apoptosis-related proteins were measured by Western blotting.
RESULTS
Baicalin significantly increased the activity of SOD, CAT and GSH-Px in colon tissue of rats with UC, but significantly decreased the content of MDA, IL-1β, MPO, PEG2 and TNF-α in colon tissue of rats with UC. In the molecular mechanism, baicalin significantly decreased the expression of cleaved-caspase3, cleaved-caspase9, Bcl-2/Bax, cyt-c, NF-kB p-65, p-IKKβ/IKKβ and p-IKBα/IKBα. Baicalin could significantly inhibit p-IKBα/IKBα content change, but had no significant effect on p-IKKβ/IKKβ.
CONCLUSION
Baicalin may have a regulating effect on IKK/IKB/NF-kB signaling pathway and apoptosis-related proteins in UC rats.
目的
本研究旨在探讨黄芩素对溃疡性结肠炎（UC）大鼠 IKK/IKB/NF-kB 信号通路及凋亡相关蛋白的影响。
方法
采用组织病理学观察和结肠组织评分法建立 UC 大鼠模型，Western blot 法检测 IKK/IKB/NF-kB 信号通路及凋亡相关蛋白。
结果
黄芩素能显著提高 UC 大鼠结肠组织中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的活性，降低 MDA、IL-1β、MPO、PEG2 和 TNF-α的含量。在分子机制方面，黄芩素能显著降低 cleaved-caspase3、cleaved-caspase9、Bcl-2/Bax、cyt-c、NF-kB p-65、p-IKKβ/IKKβ和 p-IKBα/IKBα的表达。黄芩素能显著抑制 p-IKBα/IKBα含量的变化，但对 p-IKKβ/IKKβ无明显影响。
结论
黄芩素可能通过调节 IKK/IKB/NF-kB 信号通路及凋亡相关蛋白对 UC 大鼠发挥作用。
Zotero 插件