利用 tRNA halves 作为胃癌诊断的新型生物标志物。

Using tRNA halves as novel biomarkers for the diagnosis of gastric cancer.

机构信息

Department of Biochemistry and Molecular Biology, and Zhejiang Key Laboratory of Pathophysiology, Medical School of Ningbo University, Ningbo, Zhejiang 315211, China.

Department of Gastrointestinal Surgery, Ningbo No. 1 Hospital and the Affiliated Hospital of Medical School of Ningbo University, Ningbo, Zhejiang 315010, China.

出版信息

Cancer Biomark. 2019;25(2):169-176. doi: 10.3233/CBM-182184.

DOI:10.3233/CBM-182184

PMID:31104009

Abstract

BACKGROUND

tRNA halves (tiRNAs) are produced from mature tRNAs. They have important roles both with in normal cells and cancer cells. However, the diagnostic value of tiRNAs in cancers have not yet been elucidated.

OBJECTIVE

To explore the diagnostic value of tiRNA-5034-GluTTC-2 in gastric cancer.

PATIENTS AND METHODS

Quantitative reverse transcription-polymerase chain reaction was used to detect the expression levels of tiRNA-5034-GluTTC-2 in paired gastric cancer tissues and adjacent normal tissues, plasmas from patients with gastric cancer and healthy people, and gastric cancer cell lines. Then, the relationship between its levels and clinicopathological factors of patients with gastric cancer was analyzed. A receiver operating characteristic (ROC) curve was established to predict the diagnostic value.

RESULTS

tiRNA-5034-GluTTC-2 was first found to be down-regulated in gastric cancer tissues and plasmas. Its levels were significantly associated with tumor size. The area under the ROC curve (AUC) was 0.779 and 0.835 in tissue and plasma, respectively. The sensitivity, specificity and AUC were 84.7%, 92.8%, and 0.915 when tissues and plasmas were used in combination, respectively. The overall survival rate of patients with a lower expression of tiRNA-5034-GluTTC-2 was significantly lower than those with a higher expression.

CONCLUSIONS

These results indicated that tiRNA-5034-GluTTC-2 may be a novel biomarker for the diagnosis of gastric cancer.

摘要

背景

tRNA 片段（tiRNA）由成熟的 tRNA 产生。它们在正常细胞和癌细胞中都具有重要作用。然而，tiRNA 在癌症中的诊断价值尚未阐明。

目的

探讨 tiRNA-5034-GluTTC-2 在胃癌中的诊断价值。

患者与方法

采用定量逆转录-聚合酶链反应检测配对胃癌组织和癌旁正常组织、胃癌患者和健康人血浆以及胃癌细胞系中 tiRNA-5034-GluTTC-2 的表达水平。然后，分析其水平与胃癌患者临床病理因素的关系。绘制受试者工作特征（ROC）曲线以预测诊断价值。

结果

首次发现 tiRNA-5034-GluTTC-2 在胃癌组织和血浆中下调。其水平与肿瘤大小显著相关。组织和血浆中 ROC 曲线下面积（AUC）分别为 0.779 和 0.835。当组织和血浆联合使用时，灵敏度、特异性和 AUC 分别为 84.7%、92.8%和 0.915。tiRNA-5034-GluTTC-2 低表达患者的总生存率明显低于高表达患者。