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一种新型黑腹果蝇src基因的一级序列与发育表达

Primary sequence and developmental expression of a novel Drosophila melanogaster src gene.

作者信息

Gregory R J, Kammermeyer K L, Vincent W S, Wadsworth S G

出版信息

Mol Cell Biol. 1987 Jun;7(6):2119-27. doi: 10.1128/mcb.7.6.2119-2127.1987.

DOI:10.1128/mcb.7.6.2119-2127.1987
PMID:3110602
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC365333/
Abstract

We have sequenced a cDNA clone for the Drosophila melanogaster gene Dsrc28C, a homolog of the vertebrate gene c-src. The cDNA contains a single open reading frame encoding a protein of 66 kilodaltons which contains features highly conserved within the src family of tyrosine protein kinases. Novel structural features of the Dsrc28C protein include a basic pI and a polyglycine domain near the amino terminus. Cell-free translation of in vitro-transcribed RNA yielded a protein of the predicted size which could be immunoprecipitated by anti-v-src antisera. RNA blot hybridization revealed that the gene is expressed predominantly during embryogenesis, in imaginal disks of third-instar larvae, and in adult females. In situ hybridization showed that expression in adult females is largely confined to nurse cells and developing oocytes.

摘要

我们已对果蝇（Drosophila melanogaster）基因Dsrc28C的一个cDNA克隆进行了测序，该基因是脊椎动物基因c-src的同源物。该cDNA包含一个单一的开放阅读框，编码一个66千道尔顿的蛋白质，该蛋白质具有酪氨酸蛋白激酶src家族中高度保守的特征。Dsrc28C蛋白的新结构特征包括碱性的等电点和靠近氨基末端的聚甘氨酸结构域。体外转录RNA的无细胞翻译产生了预测大小的蛋白质，该蛋白质可被抗v-src抗血清免疫沉淀。RNA印迹杂交显示，该基因主要在胚胎发育期间、三龄幼虫的成虫盘以及成年雌性果蝇中表达。原位杂交表明，成年雌性果蝇中的表达主要局限于滋养细胞和发育中的卵母细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31c0/365333/cfd7cc9ab660/molcellb00078-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31c0/365333/b4235853a8ad/molcellb00078-0096-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31c0/365333/e0938f2fb9c4/molcellb00078-0096-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31c0/365333/cfd7cc9ab660/molcellb00078-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31c0/365333/b4235853a8ad/molcellb00078-0096-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31c0/365333/e0938f2fb9c4/molcellb00078-0096-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/31c0/365333/cfd7cc9ab660/molcellb00078-0097-a.jpg

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本文引用的文献

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An improved in situ hybridization method for the detection of cellular RNAs in Drosophila tissue sections and its application for localizing transcripts of the homeotic Antennapedia gene complex.一种改良的原位杂交方法，用于检测果蝇组织切片中的细胞 RNA，及其在定位同源异形 Antennapedia 基因复合物转录本中的应用。
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The Drosophila Bruton's tyrosine kinase (Btk) homolog is required for adult survival and male genital formation.果蝇布鲁顿酪氨酸激酶（Btk）同源物是成虫存活和雄性生殖器形成所必需的。
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Phosphorylation of Enabled by the Drosophila Abelson tyrosine kinase regulates the in vivo function and protein-protein interactions of Enabled.果蝇阿贝尔森酪氨酸激酶对Enabled的磷酸化作用调节了Enabled在体内的功能及蛋白质-蛋白质相互作用。
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Identification of novel Bruton's tyrosine kinase mutations in 10 unrelated subjects with X linked agammaglobulinaemia.在10名无亲缘关系的X连锁无丙种球蛋白血症患者中鉴定新型布鲁顿酪氨酸激酶突变
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Expression of the src and abl cellular oncogenes during development of Drosophila melanogaster.
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