Simon M A, Drees B, Kornberg T, Bishop J M
Cell. 1985 Oct;42(3):831-40. doi: 10.1016/0092-8674(85)90279-x.
We have examined the coding capability and expression of the Drosophila homolog of the vertebrate proto-oncogene c-src. Sequence analysis of a cDNA clone representing the Drosophila c-src locus suggests that the gene encodes a 62 kd protein that is remarkably similar to the protein product of chicken c-src. The Drosophila c-src locus is transcribed into three mRNAs that are each regulated independently during development. Drosophila c-src RNA is abundant in embryos and pupae but rare in larvae and adults. In situ hybridization reveals that after the first 8 hr of development, c-src RNA accumulates almost exclusively in neural tissues such as the brain, ventral nerve chord, and eye-antennal discs, and in differentiating smooth muscle. We conclude that c-src may not be a mitotic signal but instead may play a role in the development of neural tissue and smooth muscle.
我们研究了脊椎动物原癌基因c-src在果蝇中的编码能力和表达情况。对代表果蝇c-src基因座的一个cDNA克隆进行序列分析表明，该基因编码一种62千道尔顿的蛋白质，它与鸡c-src的蛋白质产物非常相似。果蝇c-src基因座转录成三种mRNA，它们在发育过程中各自独立受到调控。果蝇c-src RNA在胚胎和蛹中含量丰富，但在幼虫和成虫中很少见。原位杂交显示，在发育的最初8小时后，c-src RNA几乎只在神经组织如脑、腹神经索和眼触角盘中以及在分化的平滑肌中积累。我们得出结论，c-src可能不是一种有丝分裂信号，而是可能在神经组织和平滑肌的发育中起作用。
