Cox Sarah, Noronha Leela, Herald Thomas, Bean Scott, Lee Seong-Ho, Perumal Ramasamy, Wang Weiqun, Smolensky Dmitriy
Grain Quality and Structure Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Manhattan, KS, USA.
Arthropod-Borne Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Manhattan, KS, USA.
Heliyon. 2019 May 7;5(5):e01589. doi: 10.1016/j.heliyon.2019.e01589. eCollection 2019 May.
Certain foods such as turmeric and green tea have been extensively studied for anticancer properties, while high polyphenol sorghum has not received the same attention. Some bioactive compounds in with anticancer activity have been identified, indicating the further need for research and screening methods of high polyphenol sorghum varieties. This study was aimed at improving the extraction of sorghum bioactive compounds by using food-grade solvents using ethanol and citric acid. We used three sorghum varieties and green tea (GT) as a control. The extraction methods were screened for anti-proliferative properties in HepG2 and HCT-15 cancer cell lines, using a cell viability assay. Extraction conditions were improved for anti-proliferative compounds from a high-phenolic sorghum variety (HP), sumac sorghum (CS), and GT. HP was more effective at inhibiting cell viability than CB, CS, and GT. The results demonstrate an efficient method for extracting sorghum bioactive compounds for future anticancer research using food approved ingredients.
某些食物，如姜黄和绿茶，其抗癌特性已得到广泛研究，而高多酚高粱却未受到同样的关注。已鉴定出高粱中一些具有抗癌活性的生物活性化合物，这表明对高多酚高粱品种的进一步研究和筛选方法仍有需求。本研究旨在使用食品级溶剂乙醇和柠檬酸来改进高粱生物活性化合物的提取。我们使用了三个高粱品种，并以绿茶（GT）作为对照。通过细胞活力测定法，在肝癌细胞系HepG2和结肠癌细胞系HCT - 15中筛选提取方法的抗增殖特性。针对来自高酚高粱品种（HP）、漆树高粱（CS）和GT的抗增殖化合物，改进了提取条件。HP在抑制细胞活力方面比CB、CS和GT更有效。结果表明，使用食品认可成分提取高粱生物活性化合物的方法高效，可用于未来的抗癌研究。
