Department of Nutrition and Food Science, College of Agriculture and Natural Resources, University of Maryland, College Park, MD 20742, USA.
Agricultural Research Service, U.S. Department of Agriculture, Center for Grain and Animal Health Research, Manhattan, KS 66502, USA.
Oxid Med Cell Longev. 2020 Oct 2;2020:2890536. doi: 10.1155/2020/2890536. eCollection 2020.
Human colon cancer is the third leading cause of mortality in the United States and worldwide. Chemoprevention using diet is widely accepted as a promising approach for cancer management. Numerous population studies indicate a negative correlation between the incidence of colon cancer and consumption of whole grains with a high content of bioactive phenolic compounds. In the current study, we evaluated the anticancer properties of a high phenolic sorghum bran extract prepared using 70% ethanol with 5% citric acid solvent at room temperature. A significant dose-dependent suppression of cell proliferation was observed in human colon cancer cells treated with the high phenolic sorghum bran extract. Apoptosis and S phase growth arrest were induced, while cell migration and invasion were inhibited by this treatment; these effects were accompanied by altered expression of apoptosis, cell cycle, and metastasis-regulating genes. We also found that the high phenolic sorghum bran extract stimulated DNA damage in association with induction of extracellular signal-regulated kinase (ERK) and c-Jun-NH-terminal kinase (JNK) and subsequent expression of activating transcription factor 3 (ATF3). The present study expands our understanding of the potential use of high phenolic sorghum bran to prevent human colon cancer.
人结肠癌是美国和全球范围内导致死亡的第三大主要原因。使用饮食进行化学预防被广泛认为是癌症管理的一种有前途的方法。大量的人群研究表明，结肠癌的发病率与全谷物的消费呈负相关，而全谷物含有丰富的生物活性酚类化合物。在目前的研究中，我们评估了使用 70%乙醇和 5%柠檬酸溶剂在室温下制备的高酚高粱麸提取物的抗癌特性。用人结肠癌细胞进行处理时，观察到高酚高粱麸提取物对细胞增殖具有显著的剂量依赖性抑制作用。细胞凋亡和 S 期生长停滞被诱导，而细胞迁移和侵袭被抑制；这些作用伴随着凋亡、细胞周期和转移调节基因表达的改变。我们还发现，高酚高粱麸提取物通过诱导细胞外信号调节激酶 (ERK) 和 c-Jun-NH-末端激酶 (JNK) 以及随后激活转录因子 3 (ATF3) 的表达来刺激 DNA 损伤。本研究扩展了我们对高酚高粱麸预防人结肠癌的潜在用途的理解。
