Bolognani Fantin A M, Bolognani L, Ottaviani E, Franchini A
Acta Histochem. 1987;81(2):155-62. doi: 10.1016/S0065-1281(87)80007-7.
The metabolic pathways of glucose were studied by histochemical reactions in some species of gastropods living in different habitats. The glycolytic pathway is histochemically indicated by positive results for glucose-6-phosphate isomerase, fructose-1,6-biphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase, and D-lactate dehydrogenase. The enzymes of the Krebs cycle gave different responses: isocitrate dehydrogenase and L-malate dehydrogenase were positive, whilst succinate dehydrogenase was constantly negative. Malate synthetase activity was also demonstrated. Despite L-glutamate dehydrogenase is undetectable, the presence of transaminase indicates the gluconeogenetic route. Phosphoglucomutase and glucose-6-phosphate phosphatase appear also positive. The metabolic meaning of our results were discussed.
通过组织化学反应研究了生活在不同栖息地的某些腹足纲动物体内葡萄糖的代谢途径。糖酵解途径通过葡萄糖-6-磷酸异构酶、果糖-1,6-二磷酸醛缩酶、甘油醛-3-磷酸脱氢酶和D-乳酸脱氢酶的阳性结果在组织化学上得以显示。三羧酸循环的酶给出了不同的反应：异柠檬酸脱氢酶和L-苹果酸脱氢酶呈阳性，而琥珀酸脱氢酶始终呈阴性。苹果酸合成酶活性也得到了证实。尽管未检测到L-谷氨酸脱氢酶，但转氨酶的存在表明了糖异生途径。磷酸葡萄糖变位酶和葡萄糖-6-磷酸磷酸酶也呈阳性。对我们结果的代谢意义进行了讨论。
