Corzo-Gómez Josselin, García-Cordero Julio, Montes Gómez Alfredo E, Bernal-Siria Karen, Namorado-Tónix Karime, Gutierrez-Castañeda Benito, Cedillo-Barrón Leticia
Departamento de Biomedicina Molecular CINVESTAV IPN, Av. IPN # 2508 Col, San Pedro Zacatenco, 07360, México City, Mexico.
Immunology Department (UMF) Facultad de Estudios Superiores-Iztacala, Universidad Nacional Autonoma de México, Av. de los Barrios 1, Los Reyes Iztacala, Tlalnepantla, 54090, Edo. de México, Mexico.
Protein Expr Purif. 2019 Oct;162:38-43. doi: 10.1016/j.pep.2019.05.005. Epub 2019 May 18.
The envelope (E) protein from Dengue and Zika viruses comprises three functional and structural domains (DI, DII, and DIII). Domain III induces most of the neutralizing antibodies and, as such, is considered as having the highest antigenic potential for the evaluation of population-level surveillance and for detecting past infections in both Dengue and Zika patients. The present study aimed to clone and express recombinant proteins of domain III from Dengue virus serotype 2 and from Zika virus in a prokaryotic system, as well as evaluate their immunogenicity and cross-reactivity. Both antigens were successfully purified and their antigenicity was assessed in mice. The antibodies elicited by domain III of Zika and Dengue virus antigens recognized specifically the native proteins in infected cells. Furthermore, the antigens showed a more specific immunogenic response than that of domain III proteins from Dengue virus. The generated recombinant proteins can be potentially used in subunit vaccines or for surveillance studies.
登革热病毒和寨卡病毒的包膜（E）蛋白由三个功能和结构域（DI、DII和DIII）组成。结构域III诱导产生大多数中和抗体，因此，对于评估人群水平的监测以及检测登革热和寨卡病毒患者过去的感染情况而言，它被认为具有最高的抗原潜力。本研究旨在克隆并在原核系统中表达登革热病毒2型和寨卡病毒结构域III的重组蛋白，同时评估它们的免疫原性和交叉反应性。两种抗原均成功纯化，并在小鼠中评估了它们的抗原性。寨卡病毒和登革热病毒抗原的结构域III引发的抗体能够特异性识别感染细胞中的天然蛋白。此外，与登革热病毒结构域III蛋白相比，这些抗原表现出更具特异性的免疫原性反应。所产生的重组蛋白有可能用于亚单位疫苗或监测研究。
