Zhu Aiping, Tan Hongming, Cao Lixiang
1School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275 China.
2Guangdong Province Key Laboratory for Climate Change and Natural Disaster (CCND) Studies, Sun Yat-sen University, Guangzhou, 510275 China.
3 Biotech. 2019 Jun;9(6):216. doi: 10.1007/s13205-019-1746-0. Epub 2019 May 20.
The yeasts transmitted from seeds to sprouts might be used as probiotics for host plants. To investigate the inheritable yeasts of rice plants for probiotics, the fungal internal transcribed spacer (ITS) regions (ITS1 and ITS2) in rice sprouts were analyzed by Illumina-based sequencing. The fungal genera , , , , and were revealed by both ITS1 and ITS2 sequence analysis. The endophytic yeasts were isolated from rice sprouts by yeast selective medium. Compared with the negative controls, inoculation of isolate Y3 released 2.2 folds higher concentration of free phosphate in soybean meal broth. Most of the phytase activities were located in the yeast cell interiors. The shoot lengths, shoot fresh weights, and root fresh weights of inoculated seedlings increased by 35%, 80%, and 60% compared with the control seedlings, respectively. The results suggested that the rice sprouts contained diverse phytase-producing yeasts transmitted from seeds. These yeasts might be adopted as prospective probiotics to improve rice growth by increasing phosphate utilization efficacy.
从种子传播到芽苗的酵母可作为宿主植物的益生菌。为了研究水稻植株中可遗传的益生菌酵母，通过基于Illumina的测序分析了水稻芽苗中的真菌内转录间隔区（ITS）区域（ITS1和ITS2）。通过ITS1和ITS2序列分析均揭示了真菌属、、、、和。通过酵母选择培养基从水稻芽苗中分离出内生酵母。与阴性对照相比，分离株Y3接种后在豆粕肉汤中释放的游离磷酸盐浓度高出2.2倍。大多数植酸酶活性位于酵母细胞内部。与对照幼苗相比，接种幼苗的茎长、茎鲜重和根鲜重分别增加了35%、80%和60%。结果表明，水稻芽苗含有从种子传播而来的多种产植酸酶酵母。这些酵母可能被用作潜在的益生菌，通过提高磷利用效率来促进水稻生长。
